CAJ | 학술논문

目的研究尾静脉输注间充质干细胞(MSC)对大鼠角膜移植免疫排斥反应的作用.方法实验研究.原代培养Wistar大鼠骨髓MSC,达90％融合时传代培养.制作大鼠角膜移植动物模型,以Lewis大鼠为受体,Wistar大鼠为供体,随机分为A、B、C三组.A组给予1ml不含MSC的PBS,B组术前连续3d输注MSCs,C组术后当天开始连续3d输注MSC.裂隙灯显微镜下对角膜植片进行临床观察,比较2组角膜植片的平均存活时间.A组与C组部分大鼠于术后10 d行角膜免疫组化检测角膜植片CD4、CD8、CD25和CD45的表达水平.应用Kaplan-Meier检验比较3组植片平均存活时间.免疫组化数据采用单因素方差分析及两样本均数比较的t检验.结果 C组角膜植片的存活时间为(9.9±0.69)d,与A、B组[(11.83±0.54),(16.89±1.91)d]相比均有明显延长(F=5.732;P =0.001,0.019).B组角膜植片存活时间较A组相比延长,但无统计学意义(P=0.333).C组CD4、CD8、CD25的表达水平显著低于A组(t=2.477,2.359,2.445;P=0.024,0.042,0.030).结论术后输注MSC能显著延长大鼠同种异体角膜移植片存活时间,对大鼠角膜移植排斥反应有抑制作用.
목적 연구미정맥수주간충질간세포(MSC)대대서각막이식면역배척반응적작용.방법 실험연구.원대배양Wistar대서골수MSC,체90％융합시전대배양.제작대서각막이식동물모형,이Lewis대서위수체,Wistar대서위공체,수궤분위A、B、C삼조.A조급여1ml불함MSC적PBS,B조술전련속3d수주MSCs,C조술후당천개시련속3d수주MSC.렬극등현미경하대각막식편진행림상관찰,비교2조각막식편적평균존활시간.A조여C조부분대서우술후10 d행각막면역조화검측각막식편CD4、CD8、CD25화CD45적표체수평.응용Kaplan-Meier검험비교3조식편평균존활시간.면역조화수거채용단인소방차분석급량양본균수비교적t검험.결과 C조각막식편적존활시간위(9.9±0.69)d,여A、B조[(11.83±0.54),(16.89±1.91)d]상비균유명현연장(F=5.732;P =0.001,0.019).B조각막식편존활시간교A조상비연장,단무통계학의의(P=0.333).C조CD4、CD8、CD25적표체수평현저저우A조(t=2.477,2.359,2.445;P=0.024,0.042,0.030).결론 술후수주MSC능현저연장대서동충이체각막이식편존활시간,대대서각막이식배척반응유억제작용.
Objective To investigate the effect of mesenchymal stem cells (MSC) on keratoplasty rejection in a rat mode.Methods MSC from bone marrow of Wistar rats was cultured. Corneas of Wistar rats ( donors ) were transplanted to Lewis rats ( recipients ).Transplanted rats were randomly divided into A,B,C three groups.Rats in group B were injected with MSC suspended in PBS via the tail vein continually for three days before the surgery,while rats in group C accepted similar MSC transplantation after the surgery. The rats in group A were given the same volume of PBS. Grafts were scored for corneal transparency,edema and extent of neovascularization by slit lamp observation. Expressions of CD4 、CD8 、CD25 and CD45 in corneas 10 days after transplantation were examined by immunohistochemistry. Results The survival time of the corneal grafts in group C [( 9.9 ± 0.69 ) d] was significantly prnlonged compared with that of the group A and group B [(11.83 ±0.54),(16.89 ±1.91)d] (F=5.732,P=0.001,0.019). Expression level of CD4 、CD8 、CD25 of the corneal grafts in group C was lower than that of group A and group B ( t =2.477,2.359,2.445,P =0.024,0.042,0.030).Conclusion Post-operative injection of MSCs could inhibit keratoplasty rejection and prolong the corneal allografts suivival in a rat model.