中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
2期
237-239
,共3页
张进%刘志苏%刘权焰%龚铖%刘阳%孙江阳
張進%劉誌囌%劉權燄%龔鋮%劉暘%孫江暘
장진%류지소%류권염%공성%류양%손강양
癌,肝细胞%T细胞核因子%实时荧光定量PCR
癌,肝細胞%T細胞覈因子%實時熒光定量PCR
암,간세포%T세포핵인자%실시형광정량PCR
Carcinoma,hepatocellular%Nuclear factor of T cell%Real time fluorescence quantitative polymerase chain reaction
目的 观察30例患者肝癌与邻近癌旁组织中激活的T细胞核因子(NFAT)家族基因mRNA表达的差异,探讨NFAT基因在肝癌发生发展中的作用.方法 常规抽提30例患者肝癌组织与癌旁组织中的RNA,去除基因组DNA后反转录成cDNA,应用实时荧光定量聚合酶链反应(qPCR)检测标本中NFAT家族基因mRNA表达.结果 NFAT1 mRNA ratio癌组织为0.003 034±0.002 162,癌旁组织为0.010 140±0.009 742；NFAT2 mRNA ratio癌组织为0.000 336±0.000 272,癌旁组织0.002 173±0.003 380；NFAT3 mRNA ratio癌组织为0.002 907±0.002 436,癌旁组织为0.006 427±0.004 502；NFAT4 mRNA ratio癌组织为0.004 198±0.004 787,癌旁组织为0.012 370±0.010 890；NFAT5 mRNA ratio癌组织为0.079 060±0.247 600,癌旁组织为0.282 100±0.686 900；5种NFAT家族基因mRNA水平癌组织均较癌旁组织低(P＜0.01).结论 NFAT家族5种基因的mRNA水平癌组织较癌旁组织中均降低,可能与肝癌的发生发展有关.
目的 觀察30例患者肝癌與鄰近癌徬組織中激活的T細胞覈因子(NFAT)傢族基因mRNA錶達的差異,探討NFAT基因在肝癌髮生髮展中的作用.方法 常規抽提30例患者肝癌組織與癌徬組織中的RNA,去除基因組DNA後反轉錄成cDNA,應用實時熒光定量聚閤酶鏈反應(qPCR)檢測標本中NFAT傢族基因mRNA錶達.結果 NFAT1 mRNA ratio癌組織為0.003 034±0.002 162,癌徬組織為0.010 140±0.009 742；NFAT2 mRNA ratio癌組織為0.000 336±0.000 272,癌徬組織0.002 173±0.003 380；NFAT3 mRNA ratio癌組織為0.002 907±0.002 436,癌徬組織為0.006 427±0.004 502；NFAT4 mRNA ratio癌組織為0.004 198±0.004 787,癌徬組織為0.012 370±0.010 890；NFAT5 mRNA ratio癌組織為0.079 060±0.247 600,癌徬組織為0.282 100±0.686 900；5種NFAT傢族基因mRNA水平癌組織均較癌徬組織低(P＜0.01).結論 NFAT傢族5種基因的mRNA水平癌組織較癌徬組織中均降低,可能與肝癌的髮生髮展有關.
목적 관찰30례환자간암여린근암방조직중격활적T세포핵인자(NFAT)가족기인mRNA표체적차이,탐토NFAT기인재간암발생발전중적작용.방법 상규추제30례환자간암조직여암방조직중적RNA,거제기인조DNA후반전록성cDNA,응용실시형광정량취합매련반응(qPCR)검측표본중NFAT가족기인mRNA표체.결과 NFAT1 mRNA ratio암조직위0.003 034±0.002 162,암방조직위0.010 140±0.009 742；NFAT2 mRNA ratio암조직위0.000 336±0.000 272,암방조직0.002 173±0.003 380；NFAT3 mRNA ratio암조직위0.002 907±0.002 436,암방조직위0.006 427±0.004 502；NFAT4 mRNA ratio암조직위0.004 198±0.004 787,암방조직위0.012 370±0.010 890；NFAT5 mRNA ratio암조직위0.079 060±0.247 600,암방조직위0.282 100±0.686 900；5충NFAT가족기인mRNA수평암조직균교암방조직저(P＜0.01).결론 NFAT가족5충기인적mRNA수평암조직교암방조직중균강저,가능여간암적발생발전유관.
Objective To investigate differential expression ofnuclear factor of activated T cell (NFAT) gene family members mRNA in hepatocellular carcinoma (HCC) tissues and adjacent non-tumorous tissues of thirty patients,and to explorer the possible function in the process of oncogenesis and development of cancer.Methods The RNA was extracted from cancer tissue and adjacent non-tumorous tissue of thirty patients respectively,the genome DNA was removed and the RNA was reversely transcribed to cDNA.NFAT gene family members mRNA was detected by applying Real time fluorescence quantitative polymerase chain reaction (qPCR) in tissue samples.Results The mRNA ratio of NFAT1 in cancer tissue and adjacent non-tumorous tissue was 0.003 034 ± 0.002 162 and 0.010 140 ± 0.009 742 respectively.The mRNA ratio of NFAT2 in cancer tissue and adjacent non-tumorous tissue adjacent non-tumorous tissue adjacent non-tumorous tissue was 0.000 336 ±0.000 272 and 0.002 173 ±0.003 380 respectively.The mRNA ratio of NFAT3 in cancer tissue and adjacent non-tumorous tissue was 0.002 907 ± 0.002 436 and 0.006 427 ± 0.004 502 respectively.The mRNA ratio of NFAT4 in cancer tissue and adjacent non-tumorous tissue was 0.004 198 ± 0.004 787 and 0.012 370 ± 0.010 890 respectively. The mRNA ratio of NFAT5 in cancer tissue and adjacent non-tumorous tissue was 0.079 060 ± 0.247 600 and O.282 100 ± O.686 900 respectively.The mRNA level of NFAT gene family' s five members all were lower in cancer tissue than in adjacent non-tumorous tissue ( P ＜ 0.01 ).Conclusion NFAT gene family' s five members mRNA expression is all down-regulated in cancer tissue as compared with that in adjacent non-tumorous tissues,which mav be related to oncogenesis and progression of liver cancer.
