CAJ | 학술논문

目的观察类胡萝卜素族中番茄红素对原代前列腺上皮细胞增殖和雄激素受体基因片段活性的影响.方法分离培养获得原代前列腺上皮细胞后,加入1、5、10μmol/L番茄红素溶液,免疫荧光法测定其对原代上皮细胞增殖的影响.将含有构建的雄激素受体基因片段和荧光素酶报告基因的质粒DNA导入培养中的原代细胞,转染后加入1、5、10μmol/L番茄红素溶液,通过荧光光度计的结果评价番茄红素对雄激素受体基因活性的影响.结果与细胞培养基对照组比较,加入1、5、10μmol/L番茄红素溶液后细胞增殖分别减少了3.04%、6.88%和18.42%(P<0.01).与作为番茄红素溶剂的1%四氢呋喃溶剂(THF)对照组比较,加入5、10μmol/L番茄红素溶液后细胞增殖分别减少了3.86%和15.40%(P<0.05).将上述不同浓度番茄红素溶液加入转染后的含有构建的雄激素受体基因片段和荧光素酶报告基因的质粒DNA的原代细胞后,加入1、5、10μmol/L番茄红素溶液后荧光光度计数值分别降低了52.38%、68.10%和95.24%.结论番茄红素对原代前列腺上皮细胞的增殖有显著抑制作用,它对雄激素受体基因片段的活性也有着抑制作用,并且与剂量呈正相关.
목적 관찰류호라복소족중번가홍소대원대전렬선상피세포증식화웅격소수체기인편단활성적영향.방법 분리배양획득원대전렬선상피세포후,가입1、5、10μmol/L번가홍소용액,면역형광법측정기대원대상피세포증식적영향.장함유구건적웅격소수체기인편단화형광소매보고기인적질립DNA도입배양중적원대세포,전염후가입1、5、10μmol/L번가홍소용액,통과형광광도계적결과평개번가홍소대웅격소수체기인활성적영향.결과여세포배양기대조조비교,가입1、5、10μmol/L번가홍소용액후세포증식분별감소료3.04%、6.88%화18.42%(P<0.01).여작위번가홍소용제적1%사경부남용제(THF)대조조비교,가입5、10μmol/L번가홍소용액후세포증식분별감소료3.86%화15.40%(P<0.05).장상술불동농도번가홍소용액가입전염후적함유구건적웅격소수체기인편단화형광소매보고기인적질립DNA적원대세포후,가입1、5、10μmol/L번가홍소용액후형광광도계수치분별강저료52.38%、68.10%화95.24%.결론 번가홍소대원대전렬선상피세포적증식유현저억제작용,타대웅격소수체기인편단적활성야유착억제작용,병차여제량정정상관.
Objective To evaluate the impact of lycopene on cell proliferation and androgen receptor gene activity of primary prostate epithelial cells.Methods Lycopene at concentrations of 1,5,10 μmol/L was added to the isolated primary prostate epithelial cells and the impact on cell proliferation was analyzed by using immunofluorescent method.A transient transfection of a plasmid DNA recombinant containing androgene receptor element-luciferase (ARE-Luc) report gene into primary prostate epithelial cells was developed and the impact of different concentrations of lycopene on androgen receptor element was evaluated by using quantative analysis of luciferse enzyme product.Results Lycopene ( 1,5,10μmol/L)could inhibit cell growth by 3.04%,6.88% and 18.42% ( P ＜ 0.01 ) respectively as compared with PrEBM media.As compared with 1% THF solvent,the proliferation in lycopene-treated groups (5,10μmol/L) was inhibited by 3.86% and 15.40% ( P ＜0.05 ) respectively.The results of transient transfection and luminometer data showed that luminometer data were decreased by 52.38%,68.10% and 95.24% respectively when 1,5,10 μmol/L lycopene was added.Conclusion Lycopene inhibits cell proliferation and androgen receptor gene element activity in primary prostate epithelial cells in a dose-dependent manner.