CAJ | 학술논문

目的观察切除血小板源生长因子-α（PDGF-α）受体功能结构域后对血管平滑肌细胞（VSMC）增殖和凋亡的影响。方法应用基因重组技术切除（truncated）PDGF-α受体功能结构域、构建腺病毒重组 PDGF-α突变体。转染培养的人主动脉平滑肌细胞，应用荧光染色、DNA电泳及原位末端标记检测VSMC的凋亡。结果转染PDGF-α突变体经Western blot和免疫组化证实。转染PDGF-α突变体呈时间依赖性地增加VSMC的凋亡，并抑制VSMC增殖。转染PDGF-α突变体后能特异地阻断PDGF的作用，但不能阻断胰岛素的作用。结论切除PDGF-α受体功能结构域可通过增加VSMC凋亡抑制VSMC增殖。
목적관찰절제혈소판원생장인자-α（PDGF-α）수체공능결구역후대혈관평활기세포（VSMC）증식화조망적영향。방법응용기인중조기술절제（truncated）PDGF-α수체공능결구역、구건선병독중조 PDGF-α돌변체。전염배양적인주동맥평활기세포，응용형광염색、DNA전영급원위말단표기검측VSMC적조망。결과전염PDGF-α돌변체경Western blot화면역조화증실。전염PDGF-α돌변체정시간의뢰성지증가VSMC적조망，병억제VSMC증식。전염PDGF-α돌변체후능특이지조단PDGF적작용，단불능조단이도소적작용。결론절제PDGF-α수체공능결구역가통과증가VSMC조망억제VSMC증식。
Objective　To investigate the effect of truncated PDGF-α receptor on apoptosis and proliferation of cultured human vascular smooth muscle cells (VSMC). Methods　PDGF-α receptor functional domain was truncated by gene recombination, then constructed and transfected the adenoviral vector into cultured human aortic smooth muscle cells. Examine the cell apoptosis using fluorescent dye, DNA electrophoresis, and TUNEL lable. Results　The truncated PDGF-α receptor was confirmed by immnohistochemistry and Western blot. Transfecting the truncated PDGF-α receptor into VSMC resulted in time dependent increase of cell apoptosis and inhibition of VSMC proliferation. The action of PDGF was specifically blocked by transfecting truncated PDGF-α receptor and could specifically inhibit the VSMC proliferation through the increase of apoptosis, but truncated PDGF-α receptor functional domain had no effect on the action of insulin. Conclusion　Truncated PDGF-α receptor functional domain can inhibit the proliferation of aortic smooth muscle cells through the increase of apoptosis.