中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2008年
12期
725-728
,共4页
刘琳琳%李龙%陈丹%刘颖生
劉琳琳%李龍%陳丹%劉穎生
류림림%리룡%진단%류영생
藻酸盐%藻蛋白质类%膜流动性%腺苷三磷酸酶
藻痠鹽%藻蛋白質類%膜流動性%腺苷三燐痠酶
조산염%조단백질류%막류동성%선감삼린산매
Alginates%Algal proteins%Membrane fluidity%Adenosinetriphosphatase
目的 研究软骨藻酸(domoic acid,DA)对原代培养大鼠神经胶质细胞膜的损伤作用.方法 6.4×10-2、6.4×10-3,6.4×10-4 μmol/L的DA作用于原代培养的大鼠神经胶质细胞24 h后,分别测定细胞Na+-K+-ATPase和Ca2+-Mg2+ATPase的活力、细胞膜流动性和通透性的变化.结果 神经胶质细胞经DA处理后,Na+-K+-ATPase和Ca2+-Mg2+-ATPase的活力均明显受到抑制,细胞膜的流动性降低、通透性升高,低、中、高剂量DA染毒组荧光偏振度分别为0.0626±0.0051、0.0685±0.0097、0.0648±0.0086,微黏度分别为0.3154±0.0298、0.3510±0.0571、0.3286±0.0504,与对照组(荧光偏振度为0.0481±0.0069,微黏度为0.2338±0.0372)相比,差异均有统计学意义(P＜0.01).结论 DA能明显损害神经胶质细胞膜的功能,进而可能引发细胞的其他损伤.
目的 研究軟骨藻痠(domoic acid,DA)對原代培養大鼠神經膠質細胞膜的損傷作用.方法 6.4×10-2、6.4×10-3,6.4×10-4 μmol/L的DA作用于原代培養的大鼠神經膠質細胞24 h後,分彆測定細胞Na+-K+-ATPase和Ca2+-Mg2+ATPase的活力、細胞膜流動性和通透性的變化.結果 神經膠質細胞經DA處理後,Na+-K+-ATPase和Ca2+-Mg2+-ATPase的活力均明顯受到抑製,細胞膜的流動性降低、通透性升高,低、中、高劑量DA染毒組熒光偏振度分彆為0.0626±0.0051、0.0685±0.0097、0.0648±0.0086,微黏度分彆為0.3154±0.0298、0.3510±0.0571、0.3286±0.0504,與對照組(熒光偏振度為0.0481±0.0069,微黏度為0.2338±0.0372)相比,差異均有統計學意義(P＜0.01).結論 DA能明顯損害神經膠質細胞膜的功能,進而可能引髮細胞的其他損傷.
목적 연구연골조산(domoic acid,DA)대원대배양대서신경효질세포막적손상작용.방법 6.4×10-2、6.4×10-3,6.4×10-4 μmol/L적DA작용우원대배양적대서신경효질세포24 h후,분별측정세포Na+-K+-ATPase화Ca2+-Mg2+ATPase적활력、세포막류동성화통투성적변화.결과 신경효질세포경DA처리후,Na+-K+-ATPase화Ca2+-Mg2+-ATPase적활력균명현수도억제,세포막적류동성강저、통투성승고,저、중、고제량DA염독조형광편진도분별위0.0626±0.0051、0.0685±0.0097、0.0648±0.0086,미점도분별위0.3154±0.0298、0.3510±0.0571、0.3286±0.0504,여대조조(형광편진도위0.0481±0.0069,미점도위0.2338±0.0372)상비,차이균유통계학의의(P＜0.01).결론 DA능명현손해신경효질세포막적공능,진이가능인발세포적기타손상.
Objective To study the effects of domoic acid(DA)on membrane function of primary cultured rat glial cell.Methods After the glial cells were treated with 6.4×10-2,6.4×10-3 and 6.4×10-4 μmol/L DA for 24 h.the activities of Na+-K+-ATPase and Ca2+Mg2+-ATPase.the membrane fluidity and the permeability were measured to reflect the membrane funcfton.Results After treatment of DA for 24 h,the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were inhibited significantly,the membrane fluidity decreased and the membrane permeability increased.The fluorescence polarization and microviscosity in the low.middle and high dosage treatment groups were 0.0626±0.0051,0.0685±0.0097,0.0648±0.0086 and 0.3154±0.0298,0.3510±0.0571,0.3286±0.0504 respectively,compared with the control group(0.0481±0.0069 and 0.2338±0.0372)(P＜0.01).Conclusion DA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.
