CAJ | 학술논문

目的探讨E-选择索(E-selectin)及其配体在肝癌细胞与内皮细胞黏附中的作用和二者表达的临床意义;并筛选可能阻断黏附作用的药物. 方法收集78例肝细胞癌患者的临床资料,采用免疫组织化学方法检测E-selectin、sLeX、sLeA、CD44v6的表达情况,按临床特征分组并比较各组E-selectin、sLeX、sLeA、CD44v6的阳性表达率差异.体外实验采用实时荧光定量PCR检测E-selectin和细胞间黏附分子(ICAM)-1在ED25、ECV304两种内皮细胞中的表达;黏附实验检测肝癌细胞HepG2与ED25、ECV304两种内皮细胞的黏附活性,并检测不同药物对黏附作用的影响.对临床资料数据采用χ2检验、Kaplan-Meier生存分析与Wilcoxon检验;对体外实验计量数据进行方差分析.结果 E-selectin在肝癌病灶内血管内皮细胞中的阳性表达率为70.51%,sLeX、sLeA、CDJ44v6在肝癌细胞中的阳性表达率分别为64.10%、69.23%、62.90%.sLeX、sLeA、CD44v6表达阴性患者的平均生存期显著长于阳性患者(P值均<0.05).有门静脉癌栓、术前肝外转移、卫星病灶、术后3个月内复发各组E-selectin、sLeX、sLeA的阳性表达率差异均有统计学意义(P值均<0.05);而肿瘤大小、血清AFP水平均与E-selectin、sLeX、sLeA的阳性表达率无相关性;病理分级Ⅲ～Ⅳ级组与Ⅰ～Ⅱ级组的E-selectin和CD44v6的阳性表达率差异均有统计学意义(P值均<0.05).CD44v6的表达特点是术后3个月内复发者有较高的阳性率,有卫星灶者阳性率也较高.E-selectin和ICAM-1可高表达于活化后的ED25和ECV304细胞;且能介导HepG2细胞与ED25和ECV304细胞的黏附,地塞米松,丹参酮ⅡA都具有较强的抗黏附能力,且抗黏附作用随着浓度的增加而增强.结论 E-selectin、sLeX.sLeA、CD44v6的表达与患者的预后,门静脉癌栓等临床特征密切相关,可作为临床预后和术后早期复发的评估依据.E-selectin、ICAM-1及其配体可增强肝癌细胞与内皮细胞的黏附,促进肝癌转移;地塞米松、丹参酮ⅡA可阻断其黏附作用. 目的探討E-選擇索(E-selectin)及其配體在肝癌細胞與內皮細胞黏附中的作用和二者錶達的臨床意義;併篩選可能阻斷黏附作用的藥物. 方法收集78例肝細胞癌患者的臨床資料,採用免疫組織化學方法檢測E-selectin、sLeX、sLeA、CD44v6的錶達情況,按臨床特徵分組併比較各組E-selectin、sLeX、sLeA、CD44v6的暘性錶達率差異.體外實驗採用實時熒光定量PCR檢測E-selectin和細胞間黏附分子(ICAM)-1在ED25、ECV304兩種內皮細胞中的錶達;黏附實驗檢測肝癌細胞HepG2與ED25、ECV304兩種內皮細胞的黏附活性,併檢測不同藥物對黏附作用的影響.對臨床資料數據採用χ2檢驗、Kaplan-Meier生存分析與Wilcoxon檢驗;對體外實驗計量數據進行方差分析.結果 E-selectin在肝癌病竈內血管內皮細胞中的暘性錶達率為70.51%,sLeX、sLeA、CDJ44v6在肝癌細胞中的暘性錶達率分彆為64.10%、69.23%、62.90%.sLeX、sLeA、CD44v6錶達陰性患者的平均生存期顯著長于暘性患者(P值均<0.05).有門靜脈癌栓、術前肝外轉移、衛星病竈、術後3箇月內複髮各組E-selectin、sLeX、sLeA的暘性錶達率差異均有統計學意義(P值均<0.05);而腫瘤大小、血清AFP水平均與E-selectin、sLeX、sLeA的暘性錶達率無相關性;病理分級Ⅲ～Ⅳ級組與Ⅰ～Ⅱ級組的E-selectin和CD44v6的暘性錶達率差異均有統計學意義(P值均<0.05).CD44v6的錶達特點是術後3箇月內複髮者有較高的暘性率,有衛星竈者暘性率也較高.E-selectin和ICAM-1可高錶達于活化後的ED25和ECV304細胞;且能介導HepG2細胞與ED25和ECV304細胞的黏附,地塞米鬆,丹參酮ⅡA都具有較彊的抗黏附能力,且抗黏附作用隨著濃度的增加而增彊.結論 E-selectin、sLeX.sLeA、CD44v6的錶達與患者的預後,門靜脈癌栓等臨床特徵密切相關,可作為臨床預後和術後早期複髮的評估依據.E-selectin、ICAM-1及其配體可增彊肝癌細胞與內皮細胞的黏附,促進肝癌轉移;地塞米鬆、丹參酮ⅡA可阻斷其黏附作用.
목적 탐토E-선택색(E-selectin)급기배체재간암세포여내피세포점부중적작용화이자표체적림상의의;병사선가능조단점부작용적약물. 방법 수집78례간세포암환자적림상자료,채용면역조직화학방법검측E-selectin、sLeX、sLeA、CD44v6적표체정황,안림상특정분조병비교각조E-selectin、sLeX、sLeA、CD44v6적양성표체솔차이.체외실험채용실시형광정량PCR검측E-selectin화세포간점부분자(ICAM)-1재ED25、ECV304량충내피세포중적표체;점부실험검측간암세포HepG2여ED25、ECV304량충내피세포적점부활성,병검측불동약물대점부작용적영향.대림상자료수거채용χ2검험、Kaplan-Meier생존분석여Wilcoxon검험;대체외실험계량수거진행방차분석.결과 E-selectin재간암병조내혈관내피세포중적양성표체솔위70.51%,sLeX、sLeA、CDJ44v6재간암세포중적양성표체솔분별위64.10%、69.23%、62.90%.sLeX、sLeA、CD44v6표체음성환자적평균생존기현저장우양성환자(P치균<0.05).유문정맥암전、술전간외전이、위성병조、술후3개월내복발각조E-selectin、sLeX、sLeA적양성표체솔차이균유통계학의의(P치균<0.05);이종류대소、혈청AFP수평균여E-selectin、sLeX、sLeA적양성표체솔무상관성;병리분급Ⅲ～Ⅳ급조여Ⅰ～Ⅱ급조적E-selectin화CD44v6적양성표체솔차이균유통계학의의(P치균<0.05).CD44v6적표체특점시술후3개월내복발자유교고적양성솔,유위성조자양성솔야교고.E-selectin화ICAM-1가고표체우활화후적ED25화ECV304세포;차능개도HepG2세포여ED25화ECV304세포적점부,지새미송,단삼동ⅡA도구유교강적항점부능력,차항점부작용수착농도적증가이증강.결론 E-selectin、sLeX.sLeA、CD44v6적표체여환자적예후,문정맥암전등림상특정밀절상관,가작위림상예후화술후조기복발적평고의거.E-selectin、ICAM-1급기배체가증강간암세포여내피세포적점부,촉진간암전이;지새미송、단삼동ⅡA가조단기점부작용.
Objective To explore the effects of E-selectin, ICAM-1 and their legends on the adhesive metastsis of hepatocellular carcinoma (HCC), and to select possible anti-adhesion drugs for hepatocelluar carcinoma metastasis. Methods 78 HCC patients were analyzed with the correlation of clinical features to the expression levels of E-selectin, sLeX, sLeA and CD44v6 in the tumor tissue. The adhesion between HepG2 and endothelial cell lines was examined by solid phase adhesion assay in vitro. Two kinds of drugs were accessed for their anti-adhesion ability. Results The positive rate of E-selectin in vascular endothelia cells adjacent to cancer nest is 70.51 %, and which of sLeX, sLeA, CD44v6 within tumor cells is 64.10%, 69.23%,62.90% respectively. The patients' life span is closely related with the positive expression of sLeX, sLeA, CD44v6 (P = 0.008,0.001,0.022). The positive expression of E-selectin, sLeX and sLeA is significantly correlated to portal vein tumor thrombus (PVTT), preoperative extrahepatic metastasis, and satellite foci, but not to the size of tumor and AFP. The level of CD44v6 expression is significantly correlated to patient's survival time. The expression levels of E-selectin and ICAM-1 are remarkably higher after ED25 and ECV304 cell lines be activated. Meanwhile the adhesive ability of HepG2 to endothelial cell is mediated. Dexamethasone, tanshinone HA are able to block this adhesion at low concentration. Conclusions The expression levels of E-selectin, sLeX, sLeA and CD44v6 are closely correlated with clinical features. E-selectin, ICAM-1 and their legends are important molecules of hepatocellular carcinoma and endothelial cells to tumor adhesive metastasis. Dexamethasone, tanshinone II A can be hopefully used as anti-adhesion drugs.