中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2008年
4期
261-263
,共3页
白静%王俊杰%张珊文%顾晋%蔡勇
白靜%王俊傑%張珊文%顧晉%蔡勇
백정%왕준걸%장산문%고진%채용
前列腺肿瘤%基因表达%细胞凋亡%碘放射性同位素%近距离放射疗法
前列腺腫瘤%基因錶達%細胞凋亡%碘放射性同位素%近距離放射療法
전렬선종류%기인표체%세포조망%전방사성동위소%근거리방사요법
Prostatic neoplasms%Gene expression%Apoptosis%Iodine radioisotopes%Brachytherapy
目的 探讨125I粒子持续低剂量率照射对前列腺癌PC-3移植瘤bel-2、bax基因表达和细胞凋亡的影响.方法 利用2枚37 MBq 125I粒子模型对前列腺癌PC-3移植瘤裸鼠进行48,96和192 h照射,用免疫组织化学方法分析bcl-2和bax基因表达情况.用原位末端标记(TUNEL)法检测凋亡率.凋亡率和bax/bcl-2比值之间的相关性采用线性相关分析.统计学处理采用SPSS 11.0软件.结果 125I粒子持续低剂量率照射48 h后bcl-2表达下降到4.00±2.00,与对照组比较差异无统计学意义(t=2.500,P=0.067);96和192 h后bcl-2表达分别下降到2.67±1.16和3.00±1.00,与对照组比较差异有统计学意义(t=4.950,3.464;P=0.008,0.026).bax表达分别上升到11.33±2.89,8.67±1.16和8.67±1.16,与对照组比较差异有统计学意义(t=3.334,4.025,5.292;P值分别为0.029,0.016和0.006).125I粒子持续低剂量率照射48 h组PC-3细胞凋亡率为22.3%,与对照组比较差异无统计学意义(P=0.404);照射96和192 h组凋亡率增加到21.7%和30.7%,与对照组比较差异有统计学意义(P=0.016,0.036).各照射组之间凋亡率差异无统计学意义(P＞0.05).125I粒子持续低剂量率照射Pc-3细胞凋亡率和bax/bcl-2比值呈正相关(r=0.784,P=0.012).结论 125I粒子持续低剂量率照射可使PC-3细胞bcl-2表达下降,bax表达上升,诱导凋亡增加,而凋亡率与bax/bcl-2比值呈正相关.125I粒子对肿瘤细胞的抑制可能通过bcl-2、bax途径诱导细胞凋亡而发挥作用.
目的 探討125I粒子持續低劑量率照射對前列腺癌PC-3移植瘤bel-2、bax基因錶達和細胞凋亡的影響.方法 利用2枚37 MBq 125I粒子模型對前列腺癌PC-3移植瘤裸鼠進行48,96和192 h照射,用免疫組織化學方法分析bcl-2和bax基因錶達情況.用原位末耑標記(TUNEL)法檢測凋亡率.凋亡率和bax/bcl-2比值之間的相關性採用線性相關分析.統計學處理採用SPSS 11.0軟件.結果 125I粒子持續低劑量率照射48 h後bcl-2錶達下降到4.00±2.00,與對照組比較差異無統計學意義(t=2.500,P=0.067);96和192 h後bcl-2錶達分彆下降到2.67±1.16和3.00±1.00,與對照組比較差異有統計學意義(t=4.950,3.464;P=0.008,0.026).bax錶達分彆上升到11.33±2.89,8.67±1.16和8.67±1.16,與對照組比較差異有統計學意義(t=3.334,4.025,5.292;P值分彆為0.029,0.016和0.006).125I粒子持續低劑量率照射48 h組PC-3細胞凋亡率為22.3%,與對照組比較差異無統計學意義(P=0.404);照射96和192 h組凋亡率增加到21.7%和30.7%,與對照組比較差異有統計學意義(P=0.016,0.036).各照射組之間凋亡率差異無統計學意義(P＞0.05).125I粒子持續低劑量率照射Pc-3細胞凋亡率和bax/bcl-2比值呈正相關(r=0.784,P=0.012).結論 125I粒子持續低劑量率照射可使PC-3細胞bcl-2錶達下降,bax錶達上升,誘導凋亡增加,而凋亡率與bax/bcl-2比值呈正相關.125I粒子對腫瘤細胞的抑製可能通過bcl-2、bax途徑誘導細胞凋亡而髮揮作用.
목적 탐토125I입자지속저제량솔조사대전렬선암PC-3이식류bel-2、bax기인표체화세포조망적영향.방법 이용2매37 MBq 125I입자모형대전렬선암PC-3이식류라서진행48,96화192 h조사,용면역조직화학방법분석bcl-2화bax기인표체정황.용원위말단표기(TUNEL)법검측조망솔.조망솔화bax/bcl-2비치지간적상관성채용선성상관분석.통계학처리채용SPSS 11.0연건.결과 125I입자지속저제량솔조사48 h후bcl-2표체하강도4.00±2.00,여대조조비교차이무통계학의의(t=2.500,P=0.067);96화192 h후bcl-2표체분별하강도2.67±1.16화3.00±1.00,여대조조비교차이유통계학의의(t=4.950,3.464;P=0.008,0.026).bax표체분별상승도11.33±2.89,8.67±1.16화8.67±1.16,여대조조비교차이유통계학의의(t=3.334,4.025,5.292;P치분별위0.029,0.016화0.006).125I입자지속저제량솔조사48 h조PC-3세포조망솔위22.3%,여대조조비교차이무통계학의의(P=0.404);조사96화192 h조조망솔증가도21.7%화30.7%,여대조조비교차이유통계학의의(P=0.016,0.036).각조사조지간조망솔차이무통계학의의(P＞0.05).125I입자지속저제량솔조사Pc-3세포조망솔화bax/bcl-2비치정정상관(r=0.784,P=0.012).결론 125I입자지속저제량솔조사가사PC-3세포bcl-2표체하강,bax표체상승,유도조망증가,이조망솔여bax/bcl-2비치정정상관.125I입자대종류세포적억제가능통과bcl-2、bax도경유도세포조망이발휘작용.
Objective The aim of this study was to investigate bcl-2,bax expression and apoptosis of continuous low-dose-rate irradiation on prostate cancer(PC)-3 transplanting tumor.Methods The expression of bcl-2 and bax associated with apoptosis between experiment and control groups were analyzed using immunohistochemistry at 48,96 and 192 h after two 125I seed sources implanting model.The correlation between apoptosis and the ratio of bax/bcl-2 was analyzed using Bi-variable linear correlation.SPSS 11.0 was used to analyse the data.Results The bcl-2 expression in experiment group began to down-regulated significantly after 125I seed irradiation for 48 h as compared with control(t=2.500,P=0.067),though it was not reached to statistical significance.At 96 and 192 h after irradiation,significantly low expression of bcl-2 were noted(t=4.950,3.464;P=0.008 and 0.026).In contrast,significantly over expression of bax was noted at 48,96 and 192 h after 125I irradiation(t=3.334,4.025,5.292;P=0.029,0.016 and 0.006).The apoptotic index(AI)for PC-3 at 48,96 and 192 h after 125I irradiation were 22.3%,21.7% and 30.7%,which was significantly higher than controls when at 96 and 192 h after 125I irradiation(P=0.016 and 0.036).Moreover,positive correlation was noted between AI and bax/bcl-2 ratio(r=0.784,P=0.012).Conclusion Low-dose-rate irradiation could down-regulate the expression of bcl-2,up-regulate the expression of bax and induced PC-3 cells apoptosis.
