CAJ | 학술논문

目的探讨系统性红斑狼疮(SEE)患者外周血Th极化状况的改变以及其受CD40/CD154和地塞米松(Dex)的影响.方法使用ELISA法检测SEE患者和对照组血浆IFN-γ和IL-10水平,分离、培养外周血单个核细胞(PBMC),应用CD40 mAb和Dex进行干预,使用ELISA法检测培养液上清IFN-γ和IL-10水平.结果 SLE患者血浆IFN-γ水平明显低于对照组(P<0.01,P<0.05),活动期组血浆IL-10水平明显高于缓解期组和对照组(均P<0.01);CD40 mAb对PBMC培养液上清IFN-γ和IL-10水平无影响(均P>0.05);Dex可使SLE患者PBMC培养液上清IFN-γ水平升高(P<0.05,P<0.01),Dex可以明显降低SEE患者和对照组PBMC培养液上清IL-10水平(均P<0.05).结论 SEE患者血浆IFN-γ水平降低、IL-10水平增高,存在Th2偏移;此种改变不受CD40/CD154影响但能被Dex抑制.
목적 탐토계통성홍반랑창(SEE)환자외주혈Th겁화상황적개변이급기수CD40/CD154화지새미송(Dex)적영향.방법 사용ELISA법검측SEE환자화대조조혈장IFN-γ화IL-10수평,분리、배양외주혈단개핵세포(PBMC),응용CD40 mAb화Dex진행간예,사용ELISA법검측배양액상청IFN-γ화IL-10수평.결과 SLE환자혈장IFN-γ수평명현저우대조조(P<0.01,P<0.05),활동기조혈장IL-10수평명현고우완해기조화대조조(균P<0.01);CD40 mAb대PBMC배양액상청IFN-γ화IL-10수평무영향(균P>0.05);Dex가사SLE환자PBMC배양액상청IFN-γ수평승고(P<0.05,P<0.01),Dex가이명현강저SEE환자화대조조PBMC배양액상청IL-10수평(균P<0.05).결론 SEE환자혈장IFN-γ수평강저、IL-10수평증고,존재Th2편이;차충개변불수CD40/CD154영향단능피Dex억제.
Objective To investigate the polarization of T helper in patients with systemic lupus erythmato-sus (SLE) and its changing caused by CD40/CD154 and dexamethasone (Dex). Methods The plasma concentrations of IFN-γ and IL-10 were meas-ured by ELISA. The cultured PBMCs from patients and healthy control were treated with CD40 mAb or Dex. The supematant concentrations of IFN-γ and IL-10 were meas- ured by ELISA. Results The plasma concentration of IFN-γ in SLE was lower than that in healthy control (P < 0.01, P < 0.05 ). The plasma concentration of IL-10 in active SLE was higher than that in inactive SLE and healthy control ( all P <0.01 ). The supernatant concentrations of IFN-γ and IL-10 were not affect- ed by CD40 mAb. Dex could increase the supernatant concentration of IFN-γ in SLE (P <0.05, P <0.01 ) and significant decrease IL-10 in three groups ( all P < 0.05 ). Conclusion There were decreased plasma concentration of IFN-γ and increased IL-10 and Th2 predominance in SLE. This predominance could not be affected by CD40/CD154 but could be affected Dex.