CAJ | 학술논문

利用芽管萌发技术( GTBM)对杨树黑斑病菌Marssonina brunnea分生孢子萌发过程中的有丝分裂过程进行了观察,M.brunnea的有丝分裂过程可以分为4个时期:前期染色质逐渐浓缩变短,中期可清楚观察得到染色体,后期姐妹染色单体发生分离并分别向两极移动,末期则可见子核的形成.另外,利用DAPI、DAPI/PI和Giemsa 3种不同的染色方法对染色体进行观察比较,结果表明:Giemsa染色法最适用于M.brunnea；在M.brunnea有丝分裂中期可清楚观察到3条染色体.结合3种染色法,比较分析染色体的绝对值大小和相对值大小,其中相对值大小更加清晰和稳定地描述不同染色体间的变化.
이용아관맹발기술( GTBM)대양수흑반병균Marssonina brunnea분생포자맹발과정중적유사분렬과정진행료관찰,M.brunnea적유사분렬과정가이분위4개시기:전기염색질축점농축변단,중기가청초관찰득도염색체,후기저매염색단체발생분리병분별향량겁이동,말기칙가견자핵적형성.령외,이용DAPI、DAPI/PI화Giemsa 3충불동적염색방법대염색체진행관찰비교,결과표명:Giemsa염색법최괄용우M.brunnea；재M.brunnea유사분렬중기가청초관찰도3조염색체.결합3충염색법,비교분석염색체적절대치대소화상대치대소,기중상대치대소경가청석화은정지묘술불동염색체간적변화.
Mitotic division of Marssonina brunnea was analyzed by light microscopy and the germ tube burst method (GTBM).The mitosis of M.brunnea has four phases.The chromosomes gradually shortened and thickened during prophase,and distinctly visible at metaphase.In anaphase,the chromatids separated and moved to opposite poles,at last,daughter nuclei were shaped in telophase.Three kinds of dyes were utilized to stain the chromosomes of M.brunnea,they were:DAPI,DAPI/PI and Giemsa.We found that Giemsa staining performed best that chromosome number was easily counted at the metaphase stage.Chromosomes were morphologically characterized by absolute and relative sizes.