CAJ | 학술논문

[目的]探索适合草莓未受精子房的离体培养条件。[方法]分析草莓基因型、外源激素、蔗糖浓度、低温预处理、生长环境及发育状况、光照条件6个关键因素对诱导雌核发育的影响。[结果]选择合适基因型的露地栽培的一级花蕾,外源激素添加2,4-D,蔗糖浓度6%,低温预处理48h,黑暗变温处理是草莓未受精子房离体培养获得单倍体胚囊植株的理想条件。[结论]本研究为草莓倍性育种提供了参考。
[목적]탐색괄합초매미수정자방적리체배양조건。[방법]분석초매기인형、외원격소、자당농도、저온예처리、생장배경급발육상황、광조조건6개관건인소대유도자핵발육적영향。[결과]선택합괄기인형적로지재배적일급화뢰,외원격소첨가2,4-D,자당농도6%,저온예처리48h,흑암변온처리시초매미수정자방리체배양획득단배체배낭식주적이상조건。[결론]본연구위초매배성육충제공료삼고。
[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows：the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.