安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2011年
17期
47-48,81
,共3页
赵菁%黄奋飞%林生%林文雄
趙菁%黃奮飛%林生%林文雄
조정%황강비%림생%림문웅
纤维素降解菌%鉴定%系统发育分析
纖維素降解菌%鑒定%繫統髮育分析
섬유소강해균%감정%계통발육분석
Cellulase - producing strain%Identification%Phylogenetic analysis
采用选择性培养基从不同地区土壤中筛选得到具有纤维素分解能力的菌株共65株,从中选取纤维素降解能力较高的细菌1株并对其进行表型、生理生化和分子鉴定。结果表明株菌NJ2-008属于不动杆菌属(Acinetobacter),与乙酸钙不动杆菌(Acinetobacter calcoacetirum)的序列相似度达98%。
採用選擇性培養基從不同地區土壤中篩選得到具有纖維素分解能力的菌株共65株,從中選取纖維素降解能力較高的細菌1株併對其進行錶型、生理生化和分子鑒定。結果錶明株菌NJ2-008屬于不動桿菌屬(Acinetobacter),與乙痠鈣不動桿菌(Acinetobacter calcoacetirum)的序列相似度達98%。
채용선택성배양기종불동지구토양중사선득도구유섬유소분해능력적균주공65주,종중선취섬유소강해능력교고적세균1주병대기진행표형、생리생화화분자감정。결과표명주균NJ2-008속우불동간균속(Acinetobacter),여을산개불동간균(Acinetobacter calcoacetirum)적서렬상사도체98%。
The author screened the cellulase- producing strain from the soil of different sites by the selective medium, and then identified the strain based on their phenotype, phisiological - biochemical property and molecular identification. The result showed that strain N J2-008 is closely related to Acinetobacter calcoacetirum with 98% sequence similarity. Therefore, strain NJ2 -008 was identified as the species Acinetobacte.