CAJ | 학술논문

利用积分球漫反射近红外光谱法检测了7种动物用冻干疫苗中的水分含量。采用PLS算法，全交互验证建立定量校正模型，SECV为0．0293，SEP为0．0493，R^2为0．9936。对27个样本的预测偏差范围为-0．47—0．63，相对标准偏差为0．19％～2．24％，F检验表明近红外光谱法检测动物疫苗中水分含量与卡尔费休法无明显差异，为近红外方法在动物疫苗水分含量无损检测的应用提供了依据。
이용적분구만반사근홍외광보법검측료7충동물용동간역묘중적수분함량。채용PLS산법，전교호험증건립정량교정모형，SECV위0．0293，SEP위0．0493，R^2위0．9936。대27개양본적예측편차범위위-0．47—0．63，상대표준편차위0．19％～2．24％，F검험표명근홍외광보법검측동물역묘중수분함량여잡이비휴법무명현차이，위근홍외방법재동물역묘수분함량무손검측적응용제공료의거。
The article developed determination method of water content in animal vaccines with integrate sphere diffuse reflectance NIR spectrometer, and established quantitative calibration models with PLS algorithm and full cross - validation, SECV was 0. 0293, SEP was 0. 0493 and R^2 was 0. 9936. The predicted deviation of 27 samples distributed within the range of - 0.47 - 0.63, RSD was 0.19% - 2.24%, F test indicated that NIR method and Karl Fischer method showed no significant difference in water detection of animal vaccines. The experiment provided important reference value in animal vaccines water detection with NIR method.