CAJ | 학술논문

采用刀豆蛋白A（ConA）尾静脉注射方法建立小鼠免疫性肝损伤模型,分别用低（100mg/kg）、中（200mg/kg）、高（400mg/kg）3个不同剂量大蒜多糖（GPA）对小鼠进行灌胃,灌服7d后,小鼠尾静脉注射刀豆蛋白A,采血及取肝组织,用赖氏法测定天冬氨酸转氨酶（GOT）及丙氨酸转氨酶（GPT）变化。结果模型组和多糖组与空白组比较,除高浓度多糖组差异不显著（P〉0.05）外,其它各组均差异显著（P〈0.05）,且多糖组与模型组比较差异显著（P〈0.05）,并呈现一定的量效关系。表明不同浓度的大蒜多糖均对ConA致小鼠免疫性肝损伤具有保护作用。
채용도두단백A（ConA）미정맥주사방법건립소서면역성간손상모형,분별용저（100mg/kg）、중（200mg/kg）、고（400mg/kg）3개불동제량대산다당（GPA）대소서진행관위,관복7d후,소서미정맥주사도두단백A,채혈급취간조직,용뢰씨법측정천동안산전안매（GOT）급병안산전안매（GPT）변화。결과모형조화다당조여공백조비교,제고농도다당조차이불현저（P〉0.05）외,기타각조균차이현저（P〈0.05）,차다당조여모형조비교차이현저（P〈0.05）,병정현일정적량효관계。표명불동농도적대산다당균대ConA치소서면역성간손상구유보호작용。
After administration of garlic polysaccharide at low（100mg/kg）,middle（200mg/kg）and high（400mg/kg）dosage for seven days,ConA was given to mice by intravenous injection to establish models with liver injury.Then serum samples and liver tissues were collected to detect the levels of GOT and GPT.The results showed as follows：Compared with the blank,the levels of GOT and GPT of model and polysaccharide group showed significantly difference（P0.05）with the exception of high concentrations of polysaccharide group（P0.05）,and model group were different from that of polysaccharide group（P0.05）with a dose-effect relationship.Significant difference（P0.05）was also found between the polysaccharide groups,high concentration group was close to the blank,and lowconcentrations close to the model group,while middle concentration fall in between the two.Conclusion：The garlic polysaccharides could protect mice from immune hepatic injury induced by ConA with a certain dose-effect relationship.