水产学杂志
水產學雜誌
수산학잡지
CHINESE JOURNAL OF FISHERIES
2011年
2期
1-3
,共3页
蓝色鳞鲤%染色体%核型分析%DNA含量
藍色鱗鯉%染色體%覈型分析%DNA含量
람색린리%염색체%핵형분석%DNA함량
Cyprinus carpio blue vat%chromosome%karyotype analysis%DNA content
采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备蓝色鳞鲤染色体,对100个中期分裂相记数统计,确定蓝色鳞鲤(Cyprinus carpio blue var)的染色体数为2n=100。测得核型参数按Levan等的染色体划分标准得出:蓝色鳞鲤有15对中部着丝点染色体(m);13对亚中部着丝点染色体(sin);22对端部和亚端部着丝点染色体(st,t),其染色体总臂数(NF)为156,蓝色鳞鲤核型公式为2n=30m+26sm+44st,t。采用流式细胞分析仪测定了蓝色鳞鲤的DNA含量,与鸡血细胞标准对照相比为1.73±0.10,以鸡红细胞DNA含量2.3Pg.N^-1计,则蓝色鳞鲤的体细胞DNA含量为3.99Pg.N^-1。
採用體內註射PHA和鞦水仙素,腎細胞短期培養,常規空氣榦燥法製備藍色鱗鯉染色體,對100箇中期分裂相記數統計,確定藍色鱗鯉(Cyprinus carpio blue var)的染色體數為2n=100。測得覈型參數按Levan等的染色體劃分標準得齣:藍色鱗鯉有15對中部著絲點染色體(m);13對亞中部著絲點染色體(sin);22對耑部和亞耑部著絲點染色體(st,t),其染色體總臂數(NF)為156,藍色鱗鯉覈型公式為2n=30m+26sm+44st,t。採用流式細胞分析儀測定瞭藍色鱗鯉的DNA含量,與鷄血細胞標準對照相比為1.73±0.10,以鷄紅細胞DNA含量2.3Pg.N^-1計,則藍色鱗鯉的體細胞DNA含量為3.99Pg.N^-1。
채용체내주사PHA화추수선소,신세포단기배양,상규공기간조법제비람색린리염색체,대100개중기분렬상기수통계,학정람색린리(Cyprinus carpio blue var)적염색체수위2n=100。측득핵형삼수안Levan등적염색체화분표준득출:람색린리유15대중부착사점염색체(m);13대아중부착사점염색체(sin);22대단부화아단부착사점염색체(st,t),기염색체총비수(NF)위156,람색린리핵형공식위2n=30m+26sm+44st,t。채용류식세포분석의측정료람색린리적DNA함량,여계혈세포표준대조상비위1.73±0.10,이계홍세포DNA함량2.3Pg.N^-1계,칙람색린리적체세포DNA함량위3.99Pg.N^-1。
The diploid chromosome number, karyotype, and DNA content were studied in metaphase chromosome in kidney cells and blood of blue common carp Cyprinus carpio blue var injected with PHA and colchicin by airdrying technique. It was found that the diploid chromosome number was 2n=100. The karyotype was found to be composed of 30 m, 26sm and 44st+t chromosomes, and NF=156. The diploid nucleus DNA content was measured from the somatic cell of the blue common carp using flow cytometer (Beckman cellLab Quant, made in USA) and the DNA content (2.3 pg· N^+) in erythrocytes of chick (Gallus sp) as standard. The common carp had DNA content of3.99pg·N^-1, corresponding to the result measured chromosome number as ploidy.