CAJ | 학술논문

以宁夏引种种植的33份黑核桃树种和1份本地核桃树种为试验材料,采用改进的CTAB法提取高质量模板DNA,通过24条RAPD引物利用RAPD-PCR技术系统分析材料间的遗传关系.结果表明：试验所筛选的18条RAPD引物可以用于黑核桃树种遗传资源鉴定,较为准确地将种间材料聚类;33份黑核桃材料与作为对照参试的普通核桃亲缘关系较远,参试黑核桃材料间则表现出较近的亲缘关系.
이저하인충충식적33빈흑핵도수충화1빈본지핵도수충위시험재료,채용개진적CTAB법제취고질량모판DNA,통과24조RAPD인물이용RAPD-PCR기술계통분석재료간적유전관계.결과표명：시험소사선적18조RAPD인물가이용우흑핵도수충유전자원감정,교위준학지장충간재료취류;33빈흑핵도재료여작위대조삼시적보통핵도친연관계교원,삼시흑핵도재료간칙표현출교근적친연관계.
33 pieces of Juglans nigra and 1 Juglans were used as experimental materials,the developed CTAB method was used to extract high quality DNA,and RAPD-PCR by 24 primers was employed to analyze the genetics relationship between the experimental materials.The result showed that 18 RAPD primers could be used in appraise Juglans nigra genetics resource and clustering the experimental materials correctly.Genetics relationship date showed that 33 experimental materials of Juglans nigra was farer than Juglans,nevertheless closer genetics relationship among Juglans nigra experimental materials.