基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
Genomics and Applied Biology
2011年
3期
364-371
,共8页
东方栓孔菌%漆酶%结晶紫%染料脱色%优化
東方栓孔菌%漆酶%結晶紫%染料脫色%優化
동방전공균%칠매%결정자%염료탈색%우화
Trametes orientalis%Laccase%Crystal violet%Dye decolorization%Optimization
本研究利用东方栓孔菌（Trametes orientalis）菌株Cui6300发酵所得的粗酶液,对刚果红、结晶紫、铬天青、亚甲基蓝和中性红5种染料进行了催化脱色试验,并对其中脱色效果较好的染料进行了脱色条件优化。结果表明：东方栓孔菌漆酶粗酶液对结晶紫有相对较好的脱色效果。添加2,2＇-连氮-双（3-乙基苯并噻唑-6-磺酸）（ABTS）的粗酶液对结晶紫的降解效果没有明显促进作用,因此后续试验中直接采用粗酶液。脱色条件优化结果为：最佳初始pH值6.0,最佳培养温度55℃,最佳接种量2.0%,最佳转速160r/min,最佳底物浓度160mg/L。在此条件下反应96h,脱色率可达98.45%。本试验说明东方栓孔菌在印染废水治理方面具有较好的应用前景,可以作为一种新型菌株应用于染料脱色。
本研究利用東方栓孔菌（Trametes orientalis）菌株Cui6300髮酵所得的粗酶液,對剛果紅、結晶紫、鉻天青、亞甲基藍和中性紅5種染料進行瞭催化脫色試驗,併對其中脫色效果較好的染料進行瞭脫色條件優化。結果錶明：東方栓孔菌漆酶粗酶液對結晶紫有相對較好的脫色效果。添加2,2＇-連氮-雙（3-乙基苯併噻唑-6-磺痠）（ABTS）的粗酶液對結晶紫的降解效果沒有明顯促進作用,因此後續試驗中直接採用粗酶液。脫色條件優化結果為：最佳初始pH值6.0,最佳培養溫度55℃,最佳接種量2.0%,最佳轉速160r/min,最佳底物濃度160mg/L。在此條件下反應96h,脫色率可達98.45%。本試驗說明東方栓孔菌在印染廢水治理方麵具有較好的應用前景,可以作為一種新型菌株應用于染料脫色。
본연구이용동방전공균（Trametes orientalis）균주Cui6300발효소득적조매액,대강과홍、결정자、락천청、아갑기람화중성홍5충염료진행료최화탈색시험,병대기중탈색효과교호적염료진행료탈색조건우화。결과표명：동방전공균칠매조매액대결정자유상대교호적탈색효과。첨가2,2＇-련담-쌍（3-을기분병새서-6-광산）（ABTS）적조매액대결정자적강해효과몰유명현촉진작용,인차후속시험중직접채용조매액。탈색조건우화결과위：최가초시pH치6.0,최가배양온도55℃,최가접충량2.0%,최가전속160r/min,최가저물농도160mg/L。재차조건하반응96h,탈색솔가체98.45%。본시험설명동방전공균재인염폐수치리방면구유교호적응용전경,가이작위일충신형균주응용우염료탈색。
The decolorization experiments of five different kinds of dyes（congo red,crystal violet,chromazurine,methylene blue and neutral red） were carried with the crude laccase from Trametes orientalis strain Cui 6300 in submerged fermentation.The results demonstrated that the crude laccase from Trametes orientalis had preferable decolorization capacity on crystal violet,and its optimal decolorization conditions were conducted.Moreover,the crude laccase were used in the following experiments because the crude laccase 2,2＇-azino-bis（3-ethylbenzthiazoline-6-sulphonic acid）（ABTS） system showed similar decolorization rate.The optimal initial pH value,tempera ture,inoculum,shaking speed and substrate concentration were 6.0,55 ℃,2.0%,160 r/min and 160 mg/L,respectively.Under these conditions for 96 h the decolorization rate of crystal violet was up to 98.45%.All the results indicated that the crude laccase from Trametes orientalis was able to decolorize wastewater from printing and dyeing industries,and the selected strain could be a potential candidate in the future for decolorizing wastewater which contains crystal violet.
