细粒棘球绦虫高免动物血清的特异性和敏感性分析
세립극구조충고면동물혈청적특이성화민감성분석
Echinococcus Granulosus High from Animal Serum Specificity and Sensitivity
  • 万方数据
    草食家畜 초식가축
    GRASS-FEEDING LIVESTOCK
    2011年 4期 76-79 ,共4页

    闫昊%张旭%古努尔·吐尔逊%米晓云%石保新%薛晶%巫剑%赵莉%李丹%张睿%张壮志

    염호%장욱%고노이·토이손%미효운%석보신%설정%무검%조리%리단%장예%장장지

    细粒棘球绦虫%高免血清%粪抗原%夹心法-ELISA 細粒棘毬縚蟲%高免血清%糞抗原%夾心法-ELISA
    세립극구조충%고면혈청%분항원%협심법-ELISA
    Echinococcus gran losus%High serum free%Dung antigen%Sandwich-ELlSA
    目的获得用于诊断细粒棘球绦虫成虫感染时特异性和敏感性高的抗血清。方法用细粒棘球绦虫成虫抗原免疫兔2只，制备高免血清，血清用饱和硫酸铵沉淀、透析、获得比较纯化的抗体，测量浓度和效价后，3μg／ml兔抗体包被，检测感染了不同犬带科绦虫的实验犬和空白犬粪样，单克隆抗体补捉抗原（1：6000），再用HRP标记的兔抗小鼠IgM（1：2000），OD405nm读值，用以判定兔高免血清的特异性和敏感性。，结果高免血清抗体效价均为16万，其敏感性为85％（34／40），特异性为87．5％（42／48）。结论此高免血清可以用于建立高敏感性和高特异性的诊断细粒棘球绦虫成虫感染的ELISA方法的建立和优化。
    목적획득용우진단세립극구조충성충감염시특이성화민감성고적항혈청。방법용세립극구조충성충항원면역토2지，제비고면혈청，혈청용포화류산안침정、투석、획득비교순화적항체，측량농도화효개후，3μg／ml토항체포피，검측감염료불동견대과조충적실험견화공백견분양，단극륭항체보착항원（1：6000），재용HRP표기적토항소서IgM（1：2000），OD405nm독치，용이판정토고면혈청적특이성화민감성。，결과고면혈청항체효개균위16만，기민감성위85％（34／40），특이성위87．5％（42／48）。결론차고면혈청가이용우건립고민감성화고특이성적진단세립극구조충성충감염적ELISA방법적건립화우화。
    Objective Get used to diagnose echinococcus grantxlosus adlxlt infection in the specificity and sensitivity high serum protein resistance. Methods Eehinoeoccus grantxlosus adult antigen immune rabbit 2 only,Preparation high serum free, Serum with 45% of the saturated ammonium slxlfate precipitation, dialysis, obtain purified antibodies, After measuring concentration and titer, 3μg/ml rabbit antibody bag was , Detection infected with families of different dogs experimental dogs and blank tapeworms dog dung samples , Monoelonal antibody capture antigen （1：6 000） , Garnish with the rabbit HRP markers in mice resistance IgM （1：2000）, OD 405nm read values, To determine the specificity of high rabbit serum free and sensitivity. Results The results High serum free antibody titer all be 16 million, The sensitivity is 85% （34/40）, specificity of 87.6% （42/48）.Conclusion This hyperimmune serum can be used to create high sensitivity and high specificity of the diagnosis of adult Echinococcus infection ELISA method development and optimization.
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