牛O型口蹄疫AD10新型佐剂灭活疫苗免疫持续期试验
우O형구제역AD10신형좌제멸활역묘면역지속기시험
Immunity Duration Test of Foot-and-mouth Disease Inactived Vaccines Type 0 With AD10 New Adjuvants
  • 万方数据
    草食家畜 초식가축
    GRASS-FEEDING LIVESTOCK
    2011年 4期 65-71 ,共7页

    易忠%符子华%黄炯%马文戈%胡建军%魏婕%王延%魏玉荣%胡尔马西%朱刚%徐亚萍%王宾

    역충%부자화%황형%마문과%호건군%위첩%왕연%위옥영%호이마서%주강%서아평%왕빈

    O型口蹄疫病毒%ADIO佐剂%灭活疫苗%免疫持续期 O型口蹄疫病毒%ADIO佐劑%滅活疫苗%免疫持續期
    O형구제역병독%ADIO좌제%멸활역묘%면역지속기
    foot-and-mouth disease virus type O%AD10 adjuvants%inactived vaccines%immunity duration
    对自行研发的实验室条件下制备的牛O型口蹄疫AD10新型佐剂灭活苗进行了3个批次疫苗免疫持续期试验，同时设置现行常用的油佐剂灭活苗作为对照组。其评估方法为在首免后每间隔第15天采用液相阻断ELISA试剂盒检测血清中O型口蹄疫抗体水平，并选择在一定免疫时段进行攻毒保护试验以确认疫苗的免疫效果，真实评价牛O型口蹄疫AD10新型佐剂灭活苗免疫牛的免疫持续期和对口蹄疫的抗感染能力。试验结果表明，本次试验AD10佐剂免疫组与现行常用油佐剂组免疫抗体水平和免疫持续期大致相同，对幼畜的免疫程序应在首次免疫60d后进行第二次加强免疫，免疫效果可达到6个月的免疫持续期。
    대자행연발적실험실조건하제비적우O형구제역AD10신형좌제멸활묘진행료3개비차역묘면역지속기시험，동시설치현행상용적유좌제멸활묘작위대조조。기평고방법위재수면후매간격제15천채용액상조단ELISA시제합검측혈청중O형구제역항체수평，병선택재일정면역시단진행공독보호시험이학인역묘적면역효과，진실평개우O형구제역AD10신형좌제멸활묘면역우적면역지속기화대구제역적항감염능력。시험결과표명，본차시험AD10좌제면역조여현행상용유좌제조면역항체수평화면역지속기대치상동，대유축적면역정서응재수차면역60d후진행제이차가강면역，면역효과가체도6개월적면역지속기。
    Three batches of foot-and-mouth disease inactived vaccines type O with AD10 new adjuvants to cattles were produced and their immune durations were also evaluated in this study, and control group which is the foot-and-mouth disease inactived vaccines type O with oil adjuvants were setting. The evaluation method is that every 15 days after the first inoculation,collect serum,and the level of anti-FMDV in cattles serum was tested by liquid phase blocking ELISA （lpb-Elisa）. During inoculation time interval, all cattles was challenged with foot-and-mouth disease inaetived vaccines type O with oil adjuvants to authentic assessment immunity duration and anti-infection ability of the vaccines. The results showed that the levels of immune antibody and immunity duration of immunized group with ADI0 adjuvant vaccine and immunized group with oil adjuvant vaccine are approximately same. It was concluded that re immunization on the 60th day was the best immune procedure for FMD in young stock and immunity duration of vaccine is over 6 months.
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