华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2010年
1期
38-40,44
,共4页
关键%程宗生%王健平%李德超%邓慧鑫
關鍵%程宗生%王健平%李德超%鄧慧鑫
관건%정종생%왕건평%리덕초%산혜흠
基因表达%信号转导%离心力%成骨细胞%骨形态发生蛋白
基因錶達%信號轉導%離心力%成骨細胞%骨形態髮生蛋白
기인표체%신호전도%리심력%성골세포%골형태발생단백
gene expression%signal transduction%centrifugation%osteoblasts%bone morphogenetic protein
目的 研究机械离心力刺激对MC3T3-E1细胞中Runx2表达的影响,探讨成骨细胞将力学刺激转化为生化响应过程中骨形态发生蛋白(BMP)信号转导通路的作用.方法 将MC3T3-E1细胞分成A、B、C、D组,分别使用含10%胎牛血清、10%胎牛血清、100 ng·mL~(-1) Nossin和100 ng·mL~(-1) Noggin的DEME培养基预处理24 h,对B、D组加载271xg离心力5 min,A、C组不进行离心加力,其余条件相同.30 min后4组分别同时收获细胞,提取总RNA,并逆转录为cDNA,通过实时荧光定量PCR检测Runx2基因表达情况.结果 B组Runx2 mRNA表达较A组明显增高(P<0.05),D组Runx2mRNA表达比B组表达明显降低(P<0.05),A、C、D组间表达水平比较差异无统计学意义(P=0.692).结论 BMP信号转导通路参与成骨细胞对机械离心力刺激的响应过程,并可能在成骨细胞对力学信号引起的信息传递级联反应中扮演重要角色.
目的 研究機械離心力刺激對MC3T3-E1細胞中Runx2錶達的影響,探討成骨細胞將力學刺激轉化為生化響應過程中骨形態髮生蛋白(BMP)信號轉導通路的作用.方法 將MC3T3-E1細胞分成A、B、C、D組,分彆使用含10%胎牛血清、10%胎牛血清、100 ng·mL~(-1) Nossin和100 ng·mL~(-1) Noggin的DEME培養基預處理24 h,對B、D組加載271xg離心力5 min,A、C組不進行離心加力,其餘條件相同.30 min後4組分彆同時收穫細胞,提取總RNA,併逆轉錄為cDNA,通過實時熒光定量PCR檢測Runx2基因錶達情況.結果 B組Runx2 mRNA錶達較A組明顯增高(P<0.05),D組Runx2mRNA錶達比B組錶達明顯降低(P<0.05),A、C、D組間錶達水平比較差異無統計學意義(P=0.692).結論 BMP信號轉導通路參與成骨細胞對機械離心力刺激的響應過程,併可能在成骨細胞對力學信號引起的信息傳遞級聯反應中扮縯重要角色.
목적 연구궤계리심력자격대MC3T3-E1세포중Runx2표체적영향,탐토성골세포장역학자격전화위생화향응과정중골형태발생단백(BMP)신호전도통로적작용.방법 장MC3T3-E1세포분성A、B、C、D조,분별사용함10%태우혈청、10%태우혈청、100 ng·mL~(-1) Nossin화100 ng·mL~(-1) Noggin적DEME배양기예처리24 h,대B、D조가재271xg리심력5 min,A、C조불진행리심가력,기여조건상동.30 min후4조분별동시수획세포,제취총RNA,병역전록위cDNA,통과실시형광정량PCR검측Runx2기인표체정황.결과 B조Runx2 mRNA표체교A조명현증고(P<0.05),D조Runx2mRNA표체비B조표체명현강저(P<0.05),A、C、D조간표체수평비교차이무통계학의의(P=0.692).결론 BMP신호전도통로삼여성골세포대궤계리심력자격적향응과정,병가능재성골세포대역학신호인기적신식전체급련반응중분연중요각색.
Objective To observe the expression of Runx2 in osteoblasts in response to centrifugation in vitro and discuss the function of bone morphogenetic protein (BMP) signal transduction pathway in this course. Methods Cells were divided into four groups, group A, B, C and D, pretreated with DMEM containing 10% fetal bovine serum, 10% fetal bovine serum, 100 ng·mL~(-1) Noggin and 100 ng·mL~(-1) Noggin for 24 hours separately. 271 ×g centrifugation was loaded for 5 min to these groups except group A and C, other conditions were the same. The total RNA of each group were extracted, and reversed transcription to cDNA after 30 min. The expression of Runx2 in response to cen trifugation in vitro was analyzed by quantitative real time PCR. Results The expression of Runx2 mRNA in group B was significantly higher than that in group A(P<0.05). The expression of Runx2 mRNA in group D was significantly lower than that in group B(P<0.05). There was no statistically significant difference among group A, C, D(P=0.692). Conclusion BMP signal transduction pathway plays an important role in the response of osteoblasts to mechanical stimulations. It may also play a central role in the cascade information dissemination of osteoblasts.
