CAJ | 학술논문

目的:观察苦参碱(Matrine,Mat)对体外人淋巴瘤Raij细胞增殖及细胞凋亡的影响,探讨 Mat 诱导 Raji 细胞凋亡的作用机制.方法:四甲基偶氮唑蓝(MTT)比色法观察Mat对Raji细胞增殖的影响;流式细胞术检测凋亡率;免疫细胞化学技术检测 Mat对Raji 细胞株 Bcl-2 蛋白表达的影响;RT-PCR法检测Bcl-2 mRNA表达.结果:不同浓度的 Mat 对 Raji 细胞均有一定的增殖抑制作用,且呈量效和时效关系;Mat能明显诱导Raji细胞凋亡;免疫细胞化学结果显示 Bcl-2 在实验组中的表达显著低于对照组.RT-PCR 显示 Bcl-2 mRNA 表达明显减弱,且随剂量增加越明显.结论:Mat 能抑制人淋巴瘤 Raji 细胞的增殖,促进细胞凋亡,其作用机制可能与下调 Bcl-2 表达有关.
목적:관찰고삼감(Matrine,Mat)대체외인림파류Raij세포증식급세포조망적영향,탐토 Mat 유도 Raji 세포조망적작용궤제.방법:사갑기우담서람(MTT)비색법관찰Mat대Raji세포증식적영향;류식세포술검측조망솔;면역세포화학기술검측 Mat대Raji 세포주 Bcl-2 단백표체적영향;RT-PCR법검측Bcl-2 mRNA표체.결과:불동농도적 Mat 대 Raji 세포균유일정적증식억제작용,차정량효화시효관계;Mat능명현유도Raji세포조망;면역세포화학결과현시 Bcl-2 재실험조중적표체현저저우대조조.RT-PCR 현시 Bcl-2 mRNA 표체명현감약,차수제량증가월명현.결론:Mat 능억제인림파류 Raji 세포적증식,촉진세포조망,기작용궤제가능여하조 Bcl-2 표체유관.
Objective:To observe the effects of matrine on proliferation and apoptosis of human lymphoma cell line Raji in vitro,and to explore its mechanism.Methods:The proliferation inhibition rate of Raji cells was assayed by MTT analysis.Cell apoptosis rate was evaluated by flow cytometry.The expression level of Bcl-2 protein was evaluated by streptavidin-biotin-peroxidase method.The Bcl-2 mRNA expression of Raji cells treated with matrine was assayed by RT-PCR.Results:The matrine of different concentrations all had cytotoxic effects on Raji cells.in a dose-and time-dependent manner.The apoptosis induced by matrine was confirmed in Raji cells.Immunocytochemistry assessment showed the expression of Bcl-2 was significantly down-regulated after treated with matrine.The Bcl-2 mRNA expression in Raji cells was decreased after treated by matrine,with a concentration-effect relationship.Conclusion:Matrine can inhibit the proliferation of Raji cells significantly.By down-regulating the expression of Bcl-2 and inducing the apoptosis.