CAJ | 학술논문

目的:通过检测密蒙花提取物滴眼剂对去势所致干眼症大鼠泪腺凋亡相关蛋白Bax和Bcl-2表达的影响,探讨密蒙花提取物滴眼剂治疗干眼症的作用机制.方法:将45只Wistar雄性大鼠随机分为假手术组、模型组和密蒙花提取物滴眼剂治疗组(简称治疗组).模型组和治疗组大鼠行双侧去势术建立大鼠干眼症模型.治疗组大鼠用密蒙花提取物滴眼剂滴眼治疗,每次1滴,每天3次,模型组和假手术组大鼠用生理盐水滴眼.分别于治疗1、2和3个月后每组处死5只,取材用于相关指标检测.采用免疫组织化学法检测泪腺组织中凋亡相关蛋白Bax和Bcl-2的表达,并计数凋亡细胞数量.结果:治疗组治疗1、2和3个月后,泪腺导管及腺泡上皮细胞中Bax表达阳性的细胞数均明显低于模型组,Bcl-2表达阳性的细胞数均明显高于模型组,细胞凋亡数量均明显低于模型组(P＜0.01).结论:密蒙花提取物滴眼剂中主要成分为黄酮类.密蒙花提取物滴眼剂可显著抑制雄激素水平降低后大鼠干眼症的发生,抑制泪腺细胞凋亡.
목적:통과검측밀몽화제취물적안제대거세소치간안증대서루선조망상관단백Bax화Bcl-2표체적영향,탐토밀몽화제취물적안제치료간안증적작용궤제.방법:장45지Wistar웅성대서수궤분위가수술조、모형조화밀몽화제취물적안제치료조(간칭치료조).모형조화치료조대서행쌍측거세술건립대서간안증모형.치료조대서용밀몽화제취물적안제적안치료,매차1적,매천3차,모형조화가수술조대서용생리염수적안.분별우치료1、2화3개월후매조처사5지,취재용우상관지표검측.채용면역조직화학법검측루선조직중조망상관단백Bax화Bcl-2적표체,병계수조망세포수량.결과:치료조치료1、2화3개월후,루선도관급선포상피세포중Bax표체양성적세포수균명현저우모형조,Bcl-2표체양성적세포수균명현고우모형조,세포조망수량균명현저우모형조(P＜0.01).결론:밀몽화제취물적안제중주요성분위황동류.밀몽화제취물적안제가현저억제웅격소수평강저후대서간안증적발생,억제루선세포조망.
Objective: To explore the possible mechanism of eye drops of Buddleja officinalis extract in treating dry eye of castrated rats by analyzing the expressions of Bax and Bcl-2 proteins. Methods: Forty-five Wistar male rats were randomly divided into sham-operated group, untreated group and eye drops of Buddleja officinalis Maxim. extract (treatment) group. The dry eye model was established with orchiectomy in the untreated group and treatment group. Rats in the treatment group were treated with eye drops of Buddleja officinalis Maxim. extract, one drop once, three times daily. Eyes of rats in the sham-operated group and untreated group were instilled with normal saline. After one-, two-, or three-month treatment, five rats in each group were scarified respectively. Then samples were taken to detect related indices. Expressions of Bax and Bcl-2 of lacrimal gland were checked by immunohistochemical method and quantity of apoptotic cells was counted.Results: After one-, two- or three-month treatment, the quantities of expressions of Bax in acinar epithelial cells and glandular tube cells were significantly lower, and those of Bcl-2 were significantly higher in the treatment group than in the untreated group, and the quantities of apoptotic cells of the treatment group were significantly lower than those of the untreated group (P<0.01). Conclusion: The main components of extract of Buddleja officinalis Maxim. are flavonoids, which can significantly inhibit cell apoptosis in lacrimal gland.