CAJ | 학술논문

目的:探讨变应性鼻炎(AR)患者外周血淋巴细胞 CD23、CD19 表达及其与血清总 IgE、鼻部过敏症状的关系.方法:对46例AR患者进行症状评分后,采用流式细胞术及免疫化学发光法检测其外周血淋巴细胞 CD23、CD19表达率和血清总IgE水平,并以32例健康体检者为对照.结果:①AR患者外周血 CD23~+、CD19~+及CD23~+/CD19~+淋巴细胞百分率((x-)±s)分别为11.6±1.9、22.8±3.3和10.2±1.7,分别高于对照组(P＜0.05).②CD23~+、CD19~+、CD23~+/CD19~+表达百分率与血清总IgE水平、AR症状评分之间存在正相关.③三者之中以CD23~+/CD19~+表达百分率与血清总IgE水平、AR症状评分相关性最强(r值分别为0.65和0.49.P＜0.05).④CD23~+/CD19~+与AR症状评分相关程度优于血清总IgE对应值(r=0.33,P＜0.05).结论:外周血淋巴细胞 CD23、CD19 表达参与AR发病,并可能是血清总IgE水平的影响因素,检测 CD23~+/CD19~+表达率有助于对AR病情的判断.
목적:탐토변응성비염(AR)환자외주혈림파세포 CD23、CD19 표체급기여혈청총 IgE、비부과민증상적관계.방법:대46례AR환자진행증상평분후,채용류식세포술급면역화학발광법검측기외주혈림파세포 CD23、CD19표체솔화혈청총IgE수평,병이32례건강체검자위대조.결과:①AR환자외주혈 CD23~+、CD19~+급CD23~+/CD19~+림파세포백분솔((x-)±s)분별위11.6±1.9、22.8±3.3화10.2±1.7,분별고우대조조(P＜0.05).②CD23~+、CD19~+、CD23~+/CD19~+표체백분솔여혈청총IgE수평、AR증상평분지간존재정상관.③삼자지중이CD23~+/CD19~+표체백분솔여혈청총IgE수평、AR증상평분상관성최강(r치분별위0.65화0.49.P＜0.05).④CD23~+/CD19~+여AR증상평분상관정도우우혈청총IgE대응치(r=0.33,P＜0.05).결론:외주혈림파세포 CD23、CD19 표체삼여AR발병,병가능시혈청총IgE수평적영향인소,검측 CD23~+/CD19~+표체솔유조우대AR병정적판단.
Objective:To explore the expression of CD23,CD19 on peripheral blood lymphocytes as well as its association with serum total IgE levels and nasal allergic symptoms in patients with allergic rhinitis(AR). Method:Symptom scores were evaluated in 46 AR patients,expression of CD23,CD19 on peripheral blood lymphocytes were measured by flow cytometry,and serum total IgE levels were determined by immune chemiluminescence. Thirty two healthy individuals were enrolled as controls.Result:The percentage of CD23~+,CD19~+ and CD23~+/CD19~+,on peripheral blood lymphocytes in AR patients were 11.6±1.9,22.8±3.3,10.2±1.7,respectively.Higher frequencies of CD23~+,CD19~+,and CD23~+/CD19~+ were found in AR patients compared with controls (P＜0.05).There were positive correlations between expression rates of CD23~+,CD19~+,CD23~+/CD19~+ and levels of serum total IgE,nasal allergi symptom scores,respectively.CD23~+ /CD19~+ demonstrated greater correlations with serum total IgE and nasal allergic symptom(r=0.65 and 0.49,P＜0.05)than CD23~+ and CD19~+ did.Correlation between CD23~+/CD19~+ and nasal allergic symptom scores was greater than the corresponding correlations of serum total IgE(r=0.33,P＜0.05).Conclusion:CD23 and CD19 are important factors that associated with serum total IgE in the pathogenesis of AR,Analysis on the expression of CD23~+/CD19~+ on peripheral blood lymphocytes is helpful for evaluating the severity of AR.