CAJ | 학술논문

通过测定精子的激活率、运动时间及寿命,研究了pH、盐度、离子及葡萄糖等因子变化对眼斑拟石首鱼(Csiaenops ocellatus)精子活力的影响.结果表明,眼斑拟石首鱼精液中精子平均浓度为(1.203±0.22)×10~(10)个·mL~(-1);精子激活与运动的适宜盐度范围为20～35,其中盐度25时精子的激活率、运动时间及寿命分别达94.32%、9.14min及12.55min;精子激活与运动的适宜pH为6.0～8.5,适宜的KCl、NaCl及CaCl_2溶液浓度分别为600～700 mmol·L~(-1)、600mmol·L~(-1)及400～600 mmol·L~(-1),适宜的葡萄糖溶液浓度为700～900mmol·L~(-1);精子在缺少HCO_3~-或Mg~(2+)或Ca~(2+)的人工海水中激活率与在人工海水中的激活率无显著差异,但运动时间和寿命有所缩短.
통과측정정자적격활솔、운동시간급수명,연구료pH、염도、리자급포도당등인자변화대안반의석수어(Csiaenops ocellatus)정자활력적영향.결과표명,안반의석수어정액중정자평균농도위(1.203±0.22)×10~(10)개·mL~(-1);정자격활여운동적괄의염도범위위20～35,기중염도25시정자적격활솔、운동시간급수명분별체94.32%、9.14min급12.55min;정자격활여운동적괄의pH위6.0～8.5,괄의적KCl、NaCl급CaCl_2용액농도분별위600～700 mmol·L~(-1)、600mmol·L~(-1)급400～600 mmol·L~(-1),괄의적포도당용액농도위700～900mmol·L~(-1);정자재결소HCO_3~-혹Mg~(2+)혹Ca~(2+)적인공해수중격활솔여재인공해수중적격활솔무현저차이,단운동시간화수명유소축단.
The effects of pH value, salinity, glucose and ions solutions on the sperm vitality of Csiaenops ocellatus were investigated by measuring the activation ration, moving time and life-span of sperm. The results indicated that the average density of sperm was (1.203±0.22) ×10~(10)·ml~(-1) and the suitable salinity for sperm activation and movement was 20～35, while pH value 6.0～8.5. The activation rate, moving time and life-ban of sperm reached to 94.32%, 9.14 min and 12.55 min respectively when the sperm was activated in salinity 25. The proper concentration of KCl, NaCl, CaCl_2 solutions for sperm activation and movement was 600～700 mmol·L~(-1),600 mmol·L~(-1) and 400～600 mmol·L~(-1) respectively, while the proper concentration of glucose solution was 700～900mmol·L~(-1). No significant differences were found in the activation rate when the sperm was activated by the artificial seawater lacking of HCO_3~-,Mg~(2+) or Ca~(2+), but the moving time and survival time of sperm became shorter.