CAJ | 학술논문

通过对斯达油脂酵母菌(Lipomyces starkeyi 2.1608)在不同培养基中的摇瓶发酵培养,比较研究了该菌的增殖和产胞外多糖等发酵特性.结果表明,在高浓度Mn2+(79.0mg/L MnCl2 · 4H2O )和高浓度Zn2+(0.670mg/L ZnSO4 · 7H2O )的CG培养基中,总菌数和胞外多糖产量最高,总菌数分别是LS培养基的6.9倍、W培养基的 2.4倍;胞外多糖产量分别是LS培养基的4.9倍、W培养基的1.9倍,但细胞油脂蓄积量最低.在缺Mn2+和高浓度Zn2+(0.0182mg/L ZnSO4 · 7H2O )的LS培养基中,该菌细胞增殖数量和胞外多糖产量最低,但细胞油脂蓄积量和油脂产量最高,分别为CG培养基的9.8倍和1.4倍, W培养基的6.9倍和2.4倍.在Mn2+和Zn2+浓度介于二者之间的W培养基中,该菌增殖数量和胞外多糖产量介于二者之间.在C/N一定的条件下,高浓度的Mn2+和Zn2+可促进细胞增殖和产胞外多糖,而抑制油脂合成;缺Mn2+和低浓度Zn2+可抑制细胞增殖和产胞外多糖,促进油脂合成.
통과대사체유지효모균(Lipomyces starkeyi 2.1608)재불동배양기중적요병발효배양,비교연구료해균적증식화산포외다당등발효특성.결과표명,재고농도Mn2+(79.0mg/L MnCl2 · 4H2O )화고농도Zn2+(0.670mg/L ZnSO4 · 7H2O )적CG배양기중,총균수화포외다당산량최고,총균수분별시LS배양기적6.9배、W배양기적 2.4배;포외다당산량분별시LS배양기적4.9배、W배양기적1.9배,단세포유지축적량최저.재결Mn2+화고농도Zn2+(0.0182mg/L ZnSO4 · 7H2O )적LS배양기중,해균세포증식수량화포외다당산량최저,단세포유지축적량화유지산량최고,분별위CG배양기적9.8배화1.4배, W배양기적6.9배화2.4배.재Mn2+화Zn2+농도개우이자지간적W배양기중,해균증식수량화포외다당산량개우이자지간.재C/N일정적조건하,고농도적Mn2+화Zn2+가촉진세포증식화산포외다당,이억제유지합성;결Mn2+화저농도Zn2+가억제세포증식화산포외다당,촉진유지합성.
The cell growths and fermentation characteristics of exopolysaccharide by Lipomyces starkeyi 2.1608 were investigated in shake flask containing different culture mediums.The results showed that the highest cell number and the yield of exopolysaccharides were obtained in the CG culture medium containing Mn2+ (MnCl2 · 4H2O density was 79.0mg/L) and Zn2+ (ZnSO4 · 7H2O density was 0.670mg/L).The, cells number were 6.9 times and 2.4 times as much as LS culture medium and the W culture medium, respectively.The yield of exopolysaccharides was 4.9 times and 1.9 times as much as LS culture medium and the W culture medium, respectively.However, the quantity of the lipid accumulation(mg/108cells)was lowest.The quantity of cell and the yield of exopolysaccharides were lowest in the LS culture medium containing low concentrated Zn2+ (0.018mg/L ZnSO4 · 7H2O ) and no Mn2+, however, the highest content of the lipid and the yield of lipid were achieved, which were 9.8 times and 1.4 times as much as CG culture medium, respectively, and were 6.9 times and 2.4 times as much as W culture medium, respectively.Our results indicated that under certain C/N condition, the relatively higher concentration of Mn2+ and Zn2+ could promote the cell proliferation and the production of exopolysaccharides, but suppress the lipid synthesis; the lack of Mn2+ and low concentration of Zn2+ could suppress the cell proliferation and the production of exopolysaccharides, however promote lipid synthesis.These results could provide some reference in research of regulating exopolysaccharides metabolism in yeast and exopolysaccharides production by microorganism in practice.