CAJ | 학술논문

目的　观察氧化电位水(EOW)在胃镜消毒中对乙型肝炎表面抗原(HBsAg)抗原性的破坏效果。方法　模拟现场试验用EOW与人工污染的胃液作用1 min，观察其对HBsAg的破坏作用，以PBS作为对照组。现场试验以HBsAg阳性病人用后的污染胃镜，作为消毒前对照，经去有机物处理及EOW浸泡1 min，胃镜表面涂抹采样，用ELISA检测HBsAg。结果　人工污染胃液(HBsAg平均S/N值为42.16)加入胃液量100倍的EOW作用1 min后，HBsAg检测为阴性, 对照组仍为阳性。临床污染胃镜(HBsAg平均S/N值为5.99)经清洁、消毒后HBsAg检测为阴性。结论　在清除有机物后，氧化电位水可有效的破坏污染胃镜HBsAg的抗原性。
목적　관찰양화전위수(EOW)재위경소독중대을형간염표면항원(HBsAg)항원성적파배효과。방법　모의현장시험용EOW여인공오염적위액작용1 min，관찰기대HBsAg적파배작용，이PBS작위대조조。현장시험이HBsAg양성병인용후적오염위경，작위소독전대조，경거유궤물처리급EOW침포1 min，위경표면도말채양，용ELISA검측HBsAg。결과　인공오염위액(HBsAg평균S/N치위42.16)가입위액량100배적EOW작용1 min후，HBsAg검측위음성, 대조조잉위양성。림상오염위경(HBsAg평균S/N치위5.99)경청길、소독후HBsAg검측위음성。결론　재청제유궤물후，양화전위수가유효적파배오염위경HBsAg적항원성。
Objective　Observation on the effect of disinfection on gastroscope, contaminated by hepatitis B surface antigen (HBsAg) in the electrolyzed oxidizing water(EOW). Methods　Contaminated gastric juice and serum was added to EOW for 1 minute. Positive control samples were treated with PBS instead of EOW in the same way. Gastroscopes used for hepatitis patients were immersed in the EOW for 1 minute after cleaning. Samples were collected before and after treatment. ELISA was used to test HBsAg. Results　With mixed samples (average S/N=42.16) of EOW， HBsAg became negative when diluted in 100 times. However, the HBsAg of positive control samples remained positive. After cleaning the gastroscope (average S/N=5.99) immersed in EOW, HBsAg became negative. Conclusion　EOW was effective in destroying HBsAg which could be used for gastroscope disinfection.