CAJ | 학술논문

目的研究补骨脂素对MCF-7/ADR耐药细胞逆转作用及对耐药细胞内Ca2+浓度影响,探讨其可能作用机制.方法采用MTT法测定补骨脂素对细胞增殖抑制作用,高效液相色谱法检测细胞内ADR的浓度,激光共聚焦显微镜测定细胞内不同时段的Ca2+浓度.结果补骨脂素在1～20 μmol/L浓度下能不同程度降低ADR对MCF-7/ADR细胞的IC50,并不同程度提高细胞内ADR浓度;1～20 μmol/L补骨脂素在不同作用时段(24、48、96 h)对MCF-7/ADR细胞内Ca2+浓度与作用时间呈负相关.结论补骨脂素能逆转MCF-7/ADR细胞的MDR,其作用机制与影响耐药细胞内Ca2+浓度,故而增加细胞内ADR浓度有关.
목적 연구보골지소대MCF-7/ADR내약세포역전작용급대내약세포내Ca2+농도영향,탐토기가능작용궤제.방법 채용MTT법측정보골지소대세포증식억제작용,고효액상색보법검측세포내ADR적농도,격광공취초현미경측정세포내불동시단적Ca2+농도.결과 보골지소재1～20 μmol/L농도하능불동정도강저ADR대MCF-7/ADR세포적IC50,병불동정도제고세포내ADR농도;1～20 μmol/L보골지소재불동작용시단(24、48、96 h)대MCF-7/ADR세포내Ca2+농도여작용시간정부상관.결론 보골지소능역전MCF-7/ADR세포적MDR,기작용궤제여영향내약세포내Ca2+농도,고이증가세포내ADR농도유관.
Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. Results Psoralen from 1 to 20 μmol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.