CAJ | 학술논문

研究微波辅助法提取荔枝核黄酮类化合物的工艺,考察了提取溶剂、微波功率、溶剂用量、辐射时间、提取次数等因素对提取的影响.通过正交实验确定最佳的提取参数为:60%乙醇作为提取溶剂,微波功率700 W,料液比1∶ 25,辐射时间150 s ,提取一次.在此优化条件下用微波辅助,黄酮类化合物的得率为6.86%,提取物中黄酮含量达到36.7%.抗氧化性研究表明荔枝核黄酮类化合物有良好的抗氧化活性,能有效清除羟基自由基(OH·)和超氧阴离子自由基(O2-·).
연구미파보조법제취려지핵황동류화합물적공예,고찰료제취용제、미파공솔、용제용량、복사시간、제취차수등인소대제취적영향.통과정교실험학정최가적제취삼수위:60%을순작위제취용제,미파공솔700 W,료액비1∶ 25,복사시간150 s ,제취일차.재차우화조건하용미파보조,황동류화합물적득솔위6.86%,제취물중황동함량체도36.7%.항양화성연구표명려지핵황동류화합물유량호적항양화활성,능유효청제간기자유기(OH·)화초양음리자자유기(O2-·).
Microwave-assisted extract (MAE) process for flavonoids from litchi seeds was studied.Influences of solvent,microwave power,dosage of solvent,extracting time and extracting times on the extraction were investigated.From orthogonal experiments the optimum extracting parameters were determined as follows:solvents,60% alcohol;microwave power,700 W;irradiation time,150 s;material:solvents,1∶ 25 (g/mL);extracting times,one.Under these conditions the yield of flavonoids was 6.86% and the content of flavonoids in extracts reached 36.7%.Compared with the conventional extraction methods,the MAE process provided high extraction rate,high extraction selectivity and required short time.Antioxidant experiments showed that the flavonoids from litchi seeds had strong hydroxyl free radical (OH·) and superoxide anion free radical (O2-·) scavenging activities.