CAJ | 학술논문

热卡限制能使众多实验动物的寿命延长,而且它是唯一延缓哺乳动物衰老的途径.我们用原生动物阴道毛滴虫建立了热卡限制模型.在此研究体系中,该原生动物细胞周期在热卡限制的培养条件下较糖充足的培养条件下延长80%.本研究运用抑制性消减杂交的方法,以期筛选在两种培养条件下差异表达的基因,并从2个cDNA消减杂交文库中分离到15个克隆.经序列分析表明,有9个未知基因,6个已知基因.
열잡한제능사음다실험동물적수명연장,이차타시유일연완포유동물쇠로적도경.아문용원생동물음도모적충건립료열잡한제모형.재차연구체계중,해원생동물세포주기재열잡한제적배양조건하교당충족적배양조건하연장80%.본연구운용억제성소감잡교적방법,이기사선재량충배양조건하차이표체적기인,병종2개cDNA소감잡교문고중분리도15개극륭.경서렬분석표명,유9개미지기인,6개이지기인.
That calorie restriction (CR) extends lifespan has been observed in most laboratory species, and the only way to slow down aging in mammals. We established a model of CR in the protist Trichomonas vaginalis. In this system, the cell cycle of organism could be extended by reducing the concentration of glucose. Differences in gene expression are likely to explain the cell cycle extension in CRT. vaginalis. To screen differentially expressed genes in the normal and CRT.vaginalis, suppression subtractive hybridization (SSH) was utilized and 15 differentially expressed clones were isolated.Sequence analysis revealed that 9 were unknown genes which did not match to any previously reported genes, the other 6were homologues of known genes.