中国中医基础医学杂志
中國中醫基礎醫學雜誌
중국중의기출의학잡지
CHINESE JOURNAL OF BASIC MEDICINE IN ADITIONAL CHINESE MEDICINE
2001年
4期
28-32
,共5页
陈智松%吴志奎%蔡辉国%徐晶%陈玉英%吕鑫霞
陳智鬆%吳誌奎%蔡輝國%徐晶%陳玉英%呂鑫霞
진지송%오지규%채휘국%서정%진옥영%려흠하
补肾生血药%肾生髓%粒细胞-巨噬细胞集落刺激因子(GM-CSF)%逆转录-聚合酶链式反应(RT-PCR)%D-半乳糖
補腎生血藥%腎生髓%粒細胞-巨噬細胞集落刺激因子(GM-CSF)%逆轉錄-聚閤酶鏈式反應(RT-PCR)%D-半乳糖
보신생혈약%신생수%립세포-거서세포집락자격인자(GM-CSF)%역전록-취합매련식반응(RT-PCR)%D-반유당
目的:为探讨肾生髓的分子基础,阐述肾生髓的本质相关基因,我们观察了补肾生血药对衰老小鼠骨髓粒细胞-巨噬细胞集落刺激因子(GM-CSF)基因转录水平的影响。方法:采用老年小鼠和D-半乳糖致衰老小鼠两种衰老肾虚动物模型,分别连续灌服补肾生血药2个月和1个月,应用半定量逆转录-聚合酶链式反应技术(RT-PCR)检测小鼠骨髓有核细胞GM-CSF基因转录水平的变化。结果:老年小鼠和D-半乳糖致衰老小鼠骨髓有核细胞GM-CSF基因转录水平明显低于青年小鼠和正常对照小鼠,给药后则可明显得到提高。结论:补肾生血药起到滋阴补肾、益髓生血作用的分子机理之一,是促进骨髓GM-CSF基因的转录,GM-CSF是肾生髓的分子基础之一,GM-CSF基因是肾生髓的本质相关基因之一。
目的:為探討腎生髓的分子基礎,闡述腎生髓的本質相關基因,我們觀察瞭補腎生血藥對衰老小鼠骨髓粒細胞-巨噬細胞集落刺激因子(GM-CSF)基因轉錄水平的影響。方法:採用老年小鼠和D-半乳糖緻衰老小鼠兩種衰老腎虛動物模型,分彆連續灌服補腎生血藥2箇月和1箇月,應用半定量逆轉錄-聚閤酶鏈式反應技術(RT-PCR)檢測小鼠骨髓有覈細胞GM-CSF基因轉錄水平的變化。結果:老年小鼠和D-半乳糖緻衰老小鼠骨髓有覈細胞GM-CSF基因轉錄水平明顯低于青年小鼠和正常對照小鼠,給藥後則可明顯得到提高。結論:補腎生血藥起到滋陰補腎、益髓生血作用的分子機理之一,是促進骨髓GM-CSF基因的轉錄,GM-CSF是腎生髓的分子基礎之一,GM-CSF基因是腎生髓的本質相關基因之一。
목적:위탐토신생수적분자기출,천술신생수적본질상관기인,아문관찰료보신생혈약대쇠로소서골수립세포-거서세포집락자격인자(GM-CSF)기인전록수평적영향。방법:채용노년소서화D-반유당치쇠로소서량충쇠로신허동물모형,분별련속관복보신생혈약2개월화1개월,응용반정량역전록-취합매련식반응기술(RT-PCR)검측소서골수유핵세포GM-CSF기인전록수평적변화。결과:노년소서화D-반유당치쇠로소서골수유핵세포GM-CSF기인전록수평명현저우청년소서화정상대조소서,급약후칙가명현득도제고。결론:보신생혈약기도자음보신、익수생혈작용적분자궤리지일,시촉진골수GM-CSF기인적전록,GM-CSF시신생수적분자기출지일,GM-CSF기인시신생수적본질상관기인지일。
Objective: In order to explore the molecular basis of the theory that “The kidney Produces the Marrow” and clarify the essential gene related to that,the effect of Bu Shen Sheng Xue Yao (BSSXY, Traditional Chinese Medicine, ripe for tonifying the kidney to promote hematopiesis) on the level of granulocyte-macrophagocyte colony-stimulating factor (GM-CSF) gene transcription in marrow cells of aged mice was studied. Methods: After natural aged mice and D-gal's aging mice were medicated with BSSXY for two months and a month respectively,the transcription level of GM-CSF gene in marrow cells was measured by the techniques of semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR). Results: The transcription level of GM-CSF gene in the marrow cells of natural aged mice and D-gal's aging mice were significantly lower than those of young and normal control. After medication with BSSXY, they were obviously improved. Conclusions: One of the mechanisms of BSSXY's effects is to promote the transcription of GM-CSF gene. GM-CSF is one of the molecular basis of the theory that “ The kidney Produces the Marrow”. GM-CSF gene is one of the essential genes related to the theory above.