CAJ | 학술논문

目的探索混合皮肤移植中，自体皮岛诱发移植免疫耐受的免疫机制。方法在体外建立混合表皮淋巴细胞 (MELC)培养体系，以反映移植受者对皮肤移植物的免疫排斥反应。同时，将自体表皮细胞引入已建立的混合表皮淋巴细胞培养体系，以模仿混合皮肤移植中的自体皮岛效应 ,并分析发挥抑制效应的细胞来源。结果自体角质形成细胞可抑制淋巴细胞对同种异体抗原的增殖。结论自体皮岛中的角质形成细胞参与对同种异体抗原的提呈而诱导移植物耐受。
목적탐색혼합피부이식중，자체피도유발이식면역내수적면역궤제。방법재체외건립혼합표피림파세포 (MELC)배양체계，이반영이식수자대피부이식물적면역배척반응。동시，장자체표피세포인입이건립적혼합표피림파세포배양체계，이모방혼합피부이식중적자체피도효응 ,병분석발휘억제효응적세포래원。결과자체각질형성세포가억제림파세포대동충이체항원적증식。결론자체피도중적각질형성세포삼여대동충이체항원적제정이유도이식물내수。
Aim To explore the mechanism of the immune tolerance induced by the auto-islets in the intermingled skin grafting. Methods The mixed epidermal cell and lymphocyte culture (MELC) was established, in which auto-keratinocytes were added to mimic the effect exerted by the auto-islets in vivo. Results Auto-keratinocytes could inhibit the lympho-proliferation induced by alloantigen. Conclusion The immune tolerance induced by auto-islets may be related to the function of auto-keratinocytes as antigen presenting cells.