CAJ | 학술논문

目的：研究氢溴酸槟榔碱对钉螺足平滑肌的收缩活动与Ca2＋的关系，探讨其降低钉螺附壁上爬、增强灭螺效果的作用机制。方法：采用离体平滑肌实验方法，观察氢溴酸槟榔碱对足平滑肌收缩活动的影响。结果：3×10-6mol*L-1氢溴酸槟榔碱可使收缩力增加，频率加快，其收缩活动可被维拉帕米所拮抗；同等浓度的氢溴酸槟榔碱，在无Ca2＋含EGTA的Ringer's液中，亦可增强平滑肌的收缩活动，且能被咖啡因拮抗。1×10-5和3×10-5mol*L-1氢溴酸槟榔碱，均能显著地阻滞Bay K8644，增强足平滑肌的收缩活动，且阻滞作用呈浓度依赖性。结论：氢溴酸槟榔碱可能具有阻钙作用。此作用从理论上解释投药后钉螺不附壁上爬，增强灭螺效果，提供了一定的理论依据。
목적：연구경추산빈랑감대정라족평활기적수축활동여Ca2＋적관계，탐토기강저정라부벽상파、증강멸라효과적작용궤제。방법：채용리체평활기실험방법，관찰경추산빈랑감대족평활기수축활동적영향。결과：3×10-6mol*L-1경추산빈랑감가사수축력증가，빈솔가쾌，기수축활동가피유랍파미소길항；동등농도적경추산빈랑감，재무Ca2＋함EGTA적Ringer's액중，역가증강평활기적수축활동，차능피가배인길항。1×10-5화3×10-5mol*L-1경추산빈랑감，균능현저지조체Bay K8644，증강족평활기적수축활동，차조체작용정농도의뢰성。결론：경추산빈랑감가능구유조개작용。차작용종이론상해석투약후정라불부벽상파，증강멸라효과，제공료일정적이론의거。
To investigate the relationship between the effects of arecoline hydrobromide on contraction of the smooth muscles in oncomelania foot, and explore the mechanism of arecoline hydrobromide in decreasing climbing adhesion and increasing snail－killing. 　Method: The in vitro experiment was used to observe the effects of arecoline hydrobromide on contraction activitive of foot smooth muscles of oncomelania. 　Results: With a concentration of 3×10-6mol*L-1, arecoline hydrobromide strengthened the contraction and increased the frequency of the contraction of the foot smooth muscles of oncomelania, which was antagonized by verapamil. The same concentration of arecoline hydrobromide enhanced the contraction of smooth muscles in Ringer's solution with ECTA but Ca2+, and this effect was antagonized by caffeine. The concentrations of 10－5 and 3×10－5mol*L-1 of arecolin hydrobromide blocked the effect of Bay K8644 in enhancing foot smooth muscle contraction, with a characteristic of concentration－dependence. 　Conclusion:Arecoline hydrobromide may block the calcium channel of the smooth muscles in oncomelania foot. This could provide an explanation why arecoline hydrobromide decreases the rate of oncomelania climbing adhesion and enhances snail－killing.