CAJ | 학술논문

目的研究蚯蚓毒素的来源及其成分，探索其去除方法。方法对蚯蚓应激分泌液进行透析、可见及紫外分光光度法检测透析袋内外液组分、电泳分析蛋白组分、动物体内试验检测毒性。结果蚯蚓应激分泌液的蛋白组分显示剧毒性（最小致死量为0.242 mg/kg）。PAGE分离主要有两条带；蚯蚓对不同刺激产生的应激分泌液成分相同。结论蚯蚓应激分泌液的蛋白组分有毒，用加盐或微电流刺激方法可去除毒素。
목적연구구인독소적래원급기성분，탐색기거제방법。방법대구인응격분비액진행투석、가견급자외분광광도법검측투석대내외액조분、전영분석단백조분、동물체내시험검측독성。결과구인응격분비액적단백조분현시극독성（최소치사량위0.242 mg/kg）。PAGE분리주요유량조대；구인대불동자격산생적응격분비액성분상동。결론구인응격분비액적단백조분유독，용가염혹미전류자격방법가거제독소。
Purpose　The source of earthworm toxin, its ingredients and the methods to take it off were studied.Methods　The irritable secretor was dialyzed, and then examined by UV-Vis spectrum instrument.The proteins was analyzed with electrophoresis. The toxicity was tested in vivo.Results　The proteins of irritable secretor 0.242mg/kg iv cause a mouse to death.Many bands were separated from the proteins with SDS-PAGE， among which two kinds were the principal. Even with different stimulus the secretor of earthworm was identical in ingredients.Conclusion　The results demonstrated that the earthworm proteins of irritable secretion had toxicity and the toxin could be removed from earthworm by adding NaCl or 40V electricity.