CAJ | 학술논문

目的探讨溪黄草Isodonserra (Maxim.)Kudo离体培养的过程，为优良品种的快速繁殖奠定基础。方法以溪黄草无菌苗的带节茎为外植体，采用MT基本培养基，附加不同植物激素进行试验。结果培养基中附加激素BA有利于芽的发生；适宜的BA浓度为1mg/L，出芽率高达100%，且出芽数量多；基本培养基以完全的MT成分为好；生根培养基选择MT+0.2mg/LNAA,生根率为100%；试管苗移栽，成活率为90%。结论通过溪黄草离体培养的研究，获得了较高的植株再生频率，建立了有效的组织培养再生体系。
목적탐토계황초Isodonserra (Maxim.)Kudo리체배양적과정，위우량품충적쾌속번식전정기출。방법이계황초무균묘적대절경위외식체，채용MT기본배양기，부가불동식물격소진행시험。결과배양기중부가격소BA유리우아적발생；괄의적BA농도위1mg/L，출아솔고체100%，차출아수량다；기본배양기이완전적MT성분위호；생근배양기선택MT+0.2mg/LNAA,생근솔위100%；시관묘이재，성활솔위90%。결론통과계황초리체배양적연구，획득료교고적식주재생빈솔，건립료유효적조직배양재생체계。
Object To study the in vitro culture of Isodon serra (Maxim.)Kudo, so as to lay a foundatio n for the rapid propagation of its improved breeds. Methods Th e e xplants used for culture were stem segments with node from I. serra. The media were MT basal media containing different plant hormones. Results The media containing BA were effective to induce shoot format io n. Optimal BA concentration for shoot formation and growth was 1 mg/L. MT basa l medium was better than 1/2 MT. The optimal rooting medium for regenerated shoot s was MT basal medium supplemented with 0.2 mg/L NAA, a root induction rate of 1 0 0% was achieved. The survival rate of plantlet was 90% after transplanting unde r suitable condition. Conclusion An effective plant regeneration system has been established for I. serra on the basis of factors affecting the culture in vitro.