CAJ | 학술논문

目的对4个均一枸杞多糖：LBP 1a-1,LBP 1a-2,LBP 3a-1和LBP 3a-2进行结构研究和药理评价。方法采用凝胶柱色谱、酸水解、过碘酸氧化和波谱学等方法测定多糖样品的分子量、单糖组成及连接方式，并以小鼠脾淋巴细胞增殖反应为免疫活性指标对多糖样品进行活性评价。结果 4个多糖样品的分子量分别为11.5×104,9.4×104,10.3×104，8.2×104，LBP 1a-1,LBP 1a-2为1，6连接的葡聚糖；LBP 3a-1,LBP 3a-2具有1，4连接的多聚半乳糖醛酸主链，及微量的半乳糖和阿拉伯糖分支。4个多糖均具有免疫促进作用。结论 4种多糖首次从枸杞子中分离得到，具有1，4连接多聚半乳糖醛酸主链结构的多糖活性较强。
목적대4개균일구기다당：LBP 1a-1,LBP 1a-2,LBP 3a-1화LBP 3a-2진행결구연구화약리평개。방법채용응효주색보、산수해、과전산양화화파보학등방법측정다당양품적분자량、단당조성급련접방식，병이소서비림파세포증식반응위면역활성지표대다당양품진행활성평개。결과 4개다당양품적분자량분별위11.5×104,9.4×104,10.3×104，8.2×104，LBP 1a-1,LBP 1a-2위1，6련접적포취당；LBP 3a-1,LBP 3a-2구유1，4련접적다취반유당철산주련，급미량적반유당화아랍백당분지。4개다당균구유면역촉진작용。결론 4충다당수차종구기자중분리득도，구유1，4련접다취반유당철산주련결구적다당활성교강。
AIM　To investigate the structures and immunomodulation activity of four homogeneous polysaccharides: LBP 1a-1, LBP 1a-2, LBP 3a-1 and LBP 3a-2 isolated from Lycium barbarum L. brought from Zhongning County, Ningxia Province. METHODS　Their molecular weights, sugar component (constituents) and their linkages were determined by gel permeation chromatography, acid hydrolysis, periodate oxidation and NMR spectrum. The activity of immunomodulation was evaluated with splenocyte proliferation by ［3H］-TDR incorperation, in vitro. RESULTS　Four polysaccharides with molecular weights 11.5×104, 9.4×104, 10.3×104 and 8.2×104, were shown to enhance splenocyte proliferation induced by ConA. LBP 1a-1 and LBP 1a-2 were α-(1→6)-D-glucans. LBP 3a-1 and LBP 3a-2 were found to be α-(1→4)-D-polygalacturonans. CONCLUSION　The four polysaccharides were first isolated from this plant. Polysaccharides with main chain of α-(1→4)-D-polygalacturonans showed stronger immunomodulation activity.