CAJ | 학술논문

把提取的鼠脑mRNA注射到卵母细胞内，在卵母细胞上以双电极电压钳技术研究某些递质的诱导电流。实验发现除卵母细胞本身所具有的muscarine受体外，还有多种受体明显地在卵母细胞上表达。卵母细胞膜上的表达受体的反应可分平滑型、振荡型、复合型三类。其反应类型与所开启的通道及通透离子有关。平滑型反应的受体与离子通道为一复合体，激动剂开启其自身离子通道。而振荡型反应是递质通过第二信使的介导，引起胞内Ca2+库中的Ca2+释放，开启卵母细胞膜本身的Cl－通道，产生Ca2+依赖的Cl－电流,构成振荡型电流反应的主要成分。复合型二者兼有。
파제취적서뇌mRNA주사도란모세포내，재란모세포상이쌍전겁전압겸기술연구모사체질적유도전류。실험발현제란모세포본신소구유적muscarine수체외，환유다충수체명현지재란모세포상표체。란모세포막상적표체수체적반응가분평활형、진탕형、복합형삼류。기반응류형여소개계적통도급통투리자유관。평활형반응적수체여리자통도위일복합체，격동제개계기자신리자통도。이진탕형반응시체질통과제이신사적개도，인기포내Ca2+고중적Ca2+석방，개계란모세포막본신적Cl－통도，산생Ca2+의뢰적Cl－전류,구성진탕형전류반응적주요성분。복합형이자겸유。
Two-electrodes voltage-clamp technique was performed to investigate neurotransmitter-induced currents after mouse brain mRNA was injected into Xenopus oocytes. It was found that there were kinds of neurotransmitter receptors expressed in Xenopus oocytes besides native muscarine receptor in oocyte itself. The responses induced by neurotransmitters were divided into three kinds of types: oscillatory，smooth and compound. The receptor constituted a compounding with the channels in smooth response，agonist opened itself channel. The oscillatory response was mediated by second messenger，in which phosphoinositide metabolism and calcium liberation lead to the opening of chloride channels on the membrane. The compound responses had both of two responses.