基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2001年
2期
148-150
,共3页
高列%单惠敏%许荣焜%许建萍
高列%單惠敏%許榮焜%許建萍
고렬%단혜민%허영혼%허건평
褪黑激素%催乳素瘤%细胞增殖%[３H]-TdR掺入
褪黑激素%催乳素瘤%細胞增殖%[３H]-TdR摻入
퇴흑격소%최유소류%세포증식%[３H]-TdR참입
观察不同浓度的褪黑激素 (Melatonin, MLT) 对原代培养的大鼠垂体催乳素瘤细胞增殖的影响。用80～100 g体重的雄性Sprague-Dawley（SD）大鼠，皮下埋置雌二醇诱致垂体催乳素瘤（prolactinoma）形成后，取出瘤体，经消化、过滤、离心，用SFFD培养基重悬细胞，得细胞悬液，浓度为0.75×106/mL，转入24孔培养板中并加入相应剂量的MLT使其终浓度 (mol/L) 分别为10-5、 10-7 、10-9、10-11 和 10-13，另设空白对照组，于37℃、5% CO2条件下培养1h，再在每孔中加入2μCi ［3H］-TdR继续孵育24h后测counts/min值。结果显示，在对照组和各剂量（mol/L）MLT用药组10-5 , 10-7 , 10-9 , 10-11 和 10-13 的［3H］－TdR掺入率分别为 2038.75 ±186.84, 1421.75 ± 64.49, 1238.25 ± 61.72, 924.00 ± 64.44, 1033.25 ±127.22和1103.25 ±151.53 counts/min。表明10－5～10－13mol/L的MLT能有效抑制［3H］-TdR在原代培养的垂体催乳素瘤细胞中的掺入，P＜ 0.001。其中，以10－9mol/L的MLT的抑制作用最强，达54.68％。提示在培养条件下, 10－5～10－13 mol/L MLT能有效地抑制E2诱导的垂体催乳素瘤细胞的增殖。
觀察不同濃度的褪黑激素 (Melatonin, MLT) 對原代培養的大鼠垂體催乳素瘤細胞增殖的影響。用80～100 g體重的雄性Sprague-Dawley（SD）大鼠，皮下埋置雌二醇誘緻垂體催乳素瘤（prolactinoma）形成後，取齣瘤體，經消化、過濾、離心，用SFFD培養基重懸細胞，得細胞懸液，濃度為0.75×106/mL，轉入24孔培養闆中併加入相應劑量的MLT使其終濃度 (mol/L) 分彆為10-5、 10-7 、10-9、10-11 和 10-13，另設空白對照組，于37℃、5% CO2條件下培養1h，再在每孔中加入2μCi ［3H］-TdR繼續孵育24h後測counts/min值。結果顯示，在對照組和各劑量（mol/L）MLT用藥組10-5 , 10-7 , 10-9 , 10-11 和 10-13 的［3H］－TdR摻入率分彆為 2038.75 ±186.84, 1421.75 ± 64.49, 1238.25 ± 61.72, 924.00 ± 64.44, 1033.25 ±127.22和1103.25 ±151.53 counts/min。錶明10－5～10－13mol/L的MLT能有效抑製［3H］-TdR在原代培養的垂體催乳素瘤細胞中的摻入，P＜ 0.001。其中，以10－9mol/L的MLT的抑製作用最彊，達54.68％。提示在培養條件下, 10－5～10－13 mol/L MLT能有效地抑製E2誘導的垂體催乳素瘤細胞的增殖。
관찰불동농도적퇴흑격소 (Melatonin, MLT) 대원대배양적대서수체최유소류세포증식적영향。용80～100 g체중적웅성Sprague-Dawley（SD）대서，피하매치자이순유치수체최유소류（prolactinoma）형성후，취출류체，경소화、과려、리심，용SFFD배양기중현세포，득세포현액，농도위0.75×106/mL，전입24공배양판중병가입상응제량적MLT사기종농도 (mol/L) 분별위10-5、 10-7 、10-9、10-11 화 10-13，령설공백대조조，우37℃、5% CO2조건하배양1h，재재매공중가입2μCi ［3H］-TdR계속부육24h후측counts/min치。결과현시，재대조조화각제량（mol/L）MLT용약조10-5 , 10-7 , 10-9 , 10-11 화 10-13 적［3H］－TdR참입솔분별위 2038.75 ±186.84, 1421.75 ± 64.49, 1238.25 ± 61.72, 924.00 ± 64.44, 1033.25 ±127.22화1103.25 ±151.53 counts/min。표명10－5～10－13mol/L적MLT능유효억제［3H］-TdR재원대배양적수체최유소류세포중적참입，P＜ 0.001。기중，이10－9mol/L적MLT적억제작용최강，체54.68％。제시재배양조건하, 10－5～10－13 mol/L MLT능유효지억제E2유도적수체최유소류세포적증식。
In present study,we have examined the effect of melatonin at different concentrations on the proliferation of 17-β-estrogen(E2)-induced prolactinoma cells of rats.The prolactinomas were established by implanting E2-laden silastic capsule subcutaneously in 80～100 g weight Sprague-Dawley (SD) male rats.Ninety days after E2 capsules implantation,the animals were killed,and prolactinomas were removed.The suspension of tumor cells was obtained by enzymatic digestion,filtration,centrifugation,and resuspension in incubating medium.1 ml aliquot of the cell suspension containing 0.75×106 cells were distributed into each well of 24 well cell culture plate respectively.Then MLT was added to appropriate wells to make the final concentration 10-5 to 10-13 mol/L.Respective quantity of ethanol was added to control wells.Then the cells were incubated at 37℃ in 5 % CO2.After 1 h,74KBq(2μCi) / 2 μL of ［3H］-TdR was added in 50 μL of the medium.The incubation was terminated 24 h later,and then the radioactivity was counted.The result showed that the radioactivity (counts/min) of ［3H］-TdR in tumor cells in control group and test group at doses of 10-5 ,10-7 ,10-9 ,10-11 and 10-13 mol/L melatonin were 2038.75 ±186.84,1421.75 ±64.49,1238.25 ± 61.72,924 ± 64.44,1033.25± 127.22 and 1103.25 ±151.53,respectively.The results suggested that Melatonin in the concentration range 10-5～10-13 mol/L could inhibit the proliferation of 17-β-estrogen-induced rat prolactinoma cells.
