华中农业大学学报
華中農業大學學報
화중농업대학학보
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2001年
2期
119-121
,共3页
涂立超%成国英%于大胜%杨国桂
塗立超%成國英%于大勝%楊國桂
도립초%성국영%우대성%양국계
水稻%病原细菌%噬菌体
水稻%病原細菌%噬菌體
수도%병원세균%서균체
对盆栽水稻采用针刺、喷雾、盆水等不同方法接种水稻细菌性条斑病病菌后,检测稻株发病和盆水中的噬菌体,结果表明:未接种稻细条病菌的处理中,无稻株发病,盆水中也未测到噬菌体;3种接种处理均可引致稻株发病,并先后在其盆水中检测到噬菌体,说明噬菌体与致病菌有密切的伴随关系。在采用叶面喷雾和盆水接种稻白叶枯自然组合菌株B-1和单细胞系菌株Ⅳ-16试验中,B-1处理后能引起稻株发病,且在盆水中测到噬菌体;而Ⅳ-16处理后虽引起稻株发病,但盆水中无噬菌体,结果表明:在人工接种条件下植物病原细菌噬菌体是由病菌中的溶原细胞传带而来的。
對盆栽水稻採用針刺、噴霧、盆水等不同方法接種水稻細菌性條斑病病菌後,檢測稻株髮病和盆水中的噬菌體,結果錶明:未接種稻細條病菌的處理中,無稻株髮病,盆水中也未測到噬菌體;3種接種處理均可引緻稻株髮病,併先後在其盆水中檢測到噬菌體,說明噬菌體與緻病菌有密切的伴隨關繫。在採用葉麵噴霧和盆水接種稻白葉枯自然組閤菌株B-1和單細胞繫菌株Ⅳ-16試驗中,B-1處理後能引起稻株髮病,且在盆水中測到噬菌體;而Ⅳ-16處理後雖引起稻株髮病,但盆水中無噬菌體,結果錶明:在人工接種條件下植物病原細菌噬菌體是由病菌中的溶原細胞傳帶而來的。
대분재수도채용침자、분무、분수등불동방법접충수도세균성조반병병균후,검측도주발병화분수중적서균체,결과표명:미접충도세조병균적처리중,무도주발병,분수중야미측도서균체;3충접충처리균가인치도주발병,병선후재기분수중검측도서균체,설명서균체여치병균유밀절적반수관계。재채용협면분무화분수접충도백협고자연조합균주B-1화단세포계균주Ⅳ-16시험중,B-1처리후능인기도주발병,차재분수중측도서균체;이Ⅳ-16처리후수인기도주발병,단분수중무서균체,결과표명:재인공접충조건하식물병원세균서균체시유병균중적용원세포전대이래적。
5 days after the leaves of rice plants in polythere bacins were inoculating with the suspention of Xanthomonas oryzae pv. oryzicola strain by needle-pricking, spraying and pouring it in rice basins' water, the percentages of diseased plants and the quantities of phages in the rice basins' water were checked up periodically. The results showed that three kinds of inoculation methods all succeeded in causing bacterial leaf streak (BLS), the bacteriophages were detected in the rice basins' water, while there were no diseased plants and phages in non-inoculated rice basins, which indicted there was a close companionate relationship between the pathogen and the phage. When the rice plants and the rice basins' water were inoculated with the natural composite strain of B-1 and the monocell colony strain of IV-16 of X. oryzae pv. oryzae, both the bacterial leaf blight (BLB) and phages were detected in the treatment with B-l, but altnough the BLB appeared on the rice plants and no phage was detected in the treatment with Ⅳ16,which suggested that the bacteriophages were be transmitted by the lysogenic cell (cells affected by temperate phages) among the composite strain cells in the artificial inoculation condition.