CAJ | 학술논문

目的：探讨癌基因C-myc及其表达产物在肾上腺良性及恶性嗜铬细胞瘤发生中的作用。方法：应用核酸分子原位杂交及免疫组织化学方法检测29例良性肾上腺嗜铬细胞瘤及7例恶性肾上腺嗜铬细胞瘤中C-myc基因及其产物表达情况。结果：原位杂交结果显示：C-myc mRNA在良性肾上腺嗜铬细胞瘤中表达率为27.5%(8/29)，而在恶性肾上腺嗜铬细胞瘤中为71.4(5/7)(P＜0.05)；免疫组化结果显示：C-myc蛋白表达率分别为31.0%(9/29)和71.4%(5/7)(P＜0.05)；结论：在肾上腺嗜铬细胞瘤的发生过程中，C-myc基因可能对该肿瘤的恶性变起重要作用；C-myc基因产物的检测对鉴别良、恶性肾上腺嗜铬细胞瘤有一定意义。
목적：탐토암기인C-myc급기표체산물재신상선량성급악성기락세포류발생중적작용。방법：응용핵산분자원위잡교급면역조직화학방법검측29례량성신상선기락세포류급7례악성신상선기락세포류중C-myc기인급기산물표체정황。결과：원위잡교결과현시：C-myc mRNA재량성신상선기락세포류중표체솔위27.5%(8/29)，이재악성신상선기락세포류중위71.4(5/7)(P＜0.05)；면역조화결과현시：C-myc단백표체솔분별위31.0%(9/29)화71.4%(5/7)(P＜0.05)；결론：재신상선기락세포류적발생과정중，C-myc기인가능대해종류적악성변기중요작용；C-myc기인산물적검측대감별량、악성신상선기락세포류유일정의의。
To study the expression of C-myc in human adernal pheochromocytmas at protein and mRNA levels.Methods:The C-myc protein and mRNA were detected by immunohistochemistry and in situ hybridization methods in 29 cases of benign pheochromocytomas and 7 cases of malignant pheochromocytomas.Results:C-myc mRNA positive rate in 29 cases of benign and 7 cases of malignant adrenal pheochromocytomas were 27.5%(8/29) and 71.5%(5/7) C-myc protein's expression rate of benign and malignant pheochromocytomas were 31.0%(9/29) and 71.4%(5/7).Conclusion:C-myc is colsely related to the occurrence and development of malignant adrenal pheochromocytomas.The detection of C-myc in pheochromocytomas might be useful in differentiation of malignant from benign pheochromocytomas.