CAJ | 학술논문

目的　研究钙拮抗剂异搏定在离体肾保存中对肾细胞的保护作用。方法　日本纯种大耳兔，手术切取双肾，将离体肾置于4℃的 HCA灌洗液中保存24 h，检测肾细胞线粒体内总钙和肾组织三磷酸腺苷（ATP)的含量。结果　与肾脏切取后立即检测相比，保存24 h后肾细胞线粒体内钙浓度明显上升、ATP则下降；保存液中加入钙拮抗剂异博定（VP）后，上述现象可得以显著抑制。结论　在离体肾保存过程中，钙拮抗剂异博定能阻止钙进入细胞线粒体内，该现象可能具有保护离体肾的作用。
목적　연구개길항제이박정재리체신보존중대신세포적보호작용。방법　일본순충대이토，수술절취쌍신，장리체신치우4℃적 HCA관세액중보존24 h，검측신세포선립체내총개화신조직삼린산선감（ATP)적함량。결과　여신장절취후립즉검측상비，보존24 h후신세포선립체내개농도명현상승、ATP칙하강；보존액중가입개길항제이박정（VP）후，상술현상가득이현저억제。결론　재리체신보존과정중，개길항제이박정능조지개진입세포선립체내，해현상가능구유보호리체신적작용。
Objective　To investigate the protective effect of calcium antagonist Verapamil (VP) on kidney preservation in HCA solution. Methods　After kidneys were isolated from rabbits, they were perfused and stored in HCA solution or in HCA solution with VP pre-supplement at 4℃ for 24 h respectively. The contents of mitochondrial calcium in renal cells and ATP in renal tissues were measured in every group. Results　The contents of mitochondrial calcium was remarkably higher and ATP significantly lower in the kidneys in HCA solution at 4℃ for 24 h than those just after resection. But these could be inhibited in those storing in the HCA solution with VP pre-supplement. Conclusion　Calcium antagonist VP can protect kidney function during HCA solution preservation by inhibiting calcium intaking into mitochondrium.