CAJ | 학술논문

目的　观察万乃洛韦在体内抗鸭乙型肝炎病毒(DHBV)的作用，为临床治疗乙型肝炎病人提供实验依据。方法　采用重庆麻鸭乙型肝炎动物模型，用万乃洛韦治疗1个月，检测用药前后血清中的DHBV DNA及转氨酶、肝组织病理、肝组织中的DHBV DNA等。结果　万乃洛韦用药1月能使血清DHBV DNA总体水平显著降低(P<0.05或P<0.01)，各剂量组血清DHBV DNA有阴转现象。肝脏病理检查及血清转氨酶检查发现，万乃洛韦对肝组织无明显影响，大剂量用药组ALT虽有轻度升高，但停药后即恢复正常。Southern Blot检测肝组织内DHBV DNA，提示该药能抑制病毒在肝内的复制，但不能彻底清除超螺旋DNA。结论　万乃洛韦在体内有抗鸭乙型肝炎病毒的作用。
목적　관찰만내락위재체내항압을형간염병독(DHBV)적작용，위림상치료을형간염병인제공실험의거。방법　채용중경마압을형간염동물모형，용만내락위치료1개월，검측용약전후혈청중적DHBV DNA급전안매、간조직병리、간조직중적DHBV DNA등。결과　만내락위용약1월능사혈청DHBV DNA총체수평현저강저(P<0.05혹P<0.01)，각제량조혈청DHBV DNA유음전현상。간장병리검사급혈청전안매검사발현，만내락위대간조직무명현영향，대제량용약조ALT수유경도승고，단정약후즉회복정상。Southern Blot검측간조직내DHBV DNA，제시해약능억제병독재간내적복제，단불능철저청제초라선DNA。결론　만내락위재체내유항압을형간염병독적작용。
Objective　we studied the effect of the Purine mucleoside Valaciclovir on anti-duck hepatitis virus(DHBV) in vivo to provide an experimental basis for clinical treatment of patients with hepatitisB.Methods　The Chongqing duck hepatitis B virus model was treated with Valaciclovir once a day for a month at the doses of 50mg.kg－1、100mg.kg-1、200mg.kg－1of body weight per day. Serum DHBV DNA was detected four times in the course of the treatment,ALT and AST in serum and DHBV DNA in liver were detected simultaneously.Results　Valaciclovir could signsificantly lower the serum DHBV DNA level. Serum ALT of several ducks in serum rose slightly during the treatment,but became normal after 1 week stopping Valaciclovir. Examination of DHBV DNA in liver with Southern Blot indicated Valaciclovir could inhibit DHBV DNA replication,but could not completely eliminate DHBV SC DNA.Conclusion　The study confirms the safety and potent antihepaticviral activity of Valaciclovir in vivo.