中国医学科学院学报
中國醫學科學院學報
중국의학과학원학보
ACTA ACADEMIAE MEDICINAE SINICAE
2001年
1期
93-96
,共4页
鄢盛恺%任凤琴%宋耀虹%林其燧
鄢盛愷%任鳳琴%宋耀虹%林其燧
언성개%임봉금%송요홍%림기수
脂蛋白胆固醇电泳
脂蛋白膽固醇電泳
지단백담고순전영
对琼脂糖凝胶电泳法同时测定血清极低密度脂蛋白(VLDL)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)和脂蛋白(a)[LP(a)]的胆固醇[即VLDL-C、LDL-C、HDL-C和LP(a)-C]含量的方法进行评价。方法采用Helena REP全自动快速电泳系统分离血清VLDL、LDL、HDL和LP(a)，然后结合胆固醇酶染色法同时测定VLDL-C、LDL-C、HDL-C和LP(a)-C的含量。分析了该法的精密度、准确度和干扰因素，并将该法与传统方法如测定HDL-C的磷钨酸-镁沉淀法(PTA-Mg2＋法)、测定LDL-C的聚乙烯硫酸沉淀法(PVS法)及测定LP(a)的免疫透射比浊法(ITA法)进行比较。结果电泳法测定VLDL-C、LDL-C和HDL-C的批内CV分别为5.16％～7.46％、1.26％～3.28％、3.78％～5.86％；批间CV分别为8.35％～11.25％、2.78％～4.08％、4.23％～6.36％。检测线性范围总胆固醇为1.04～10.35 mmol/L，基本不受溶血、黄疸和脂血干扰。VLDL-C、LDL-C和HDL-C的平均回收率分别为90.3％、94.3％和89.6％。电泳法与传统法测定LDL-C、HDL-C的相关系数(r)分别为0.9609、0.9557。电泳法测定LP(a)-C与ITA法测定LP(a)的r为0.9235。以该法检测北京地区98例健康人，HDL-C、VLDL-C、LDL-C、LP(a)-C参考范围分别为0.95～2.10 mmol/L、0.07～0.46mmol/L、1.77～2.87 mmol/L、0～0.22 mmol/L。结论电泳法测定值与传统法一致，电泳法操作简便、快速经济，可同时测定4种脂蛋白胆固醇，适合于临床常规及研究使用。
對瓊脂糖凝膠電泳法同時測定血清極低密度脂蛋白(VLDL)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)和脂蛋白(a)[LP(a)]的膽固醇[即VLDL-C、LDL-C、HDL-C和LP(a)-C]含量的方法進行評價。方法採用Helena REP全自動快速電泳繫統分離血清VLDL、LDL、HDL和LP(a)，然後結閤膽固醇酶染色法同時測定VLDL-C、LDL-C、HDL-C和LP(a)-C的含量。分析瞭該法的精密度、準確度和榦擾因素，併將該法與傳統方法如測定HDL-C的燐鎢痠-鎂沉澱法(PTA-Mg2＋法)、測定LDL-C的聚乙烯硫痠沉澱法(PVS法)及測定LP(a)的免疫透射比濁法(ITA法)進行比較。結果電泳法測定VLDL-C、LDL-C和HDL-C的批內CV分彆為5.16％～7.46％、1.26％～3.28％、3.78％～5.86％；批間CV分彆為8.35％～11.25％、2.78％～4.08％、4.23％～6.36％。檢測線性範圍總膽固醇為1.04～10.35 mmol/L，基本不受溶血、黃疸和脂血榦擾。VLDL-C、LDL-C和HDL-C的平均迴收率分彆為90.3％、94.3％和89.6％。電泳法與傳統法測定LDL-C、HDL-C的相關繫數(r)分彆為0.9609、0.9557。電泳法測定LP(a)-C與ITA法測定LP(a)的r為0.9235。以該法檢測北京地區98例健康人，HDL-C、VLDL-C、LDL-C、LP(a)-C參攷範圍分彆為0.95～2.10 mmol/L、0.07～0.46mmol/L、1.77～2.87 mmol/L、0～0.22 mmol/L。結論電泳法測定值與傳統法一緻，電泳法操作簡便、快速經濟，可同時測定4種脂蛋白膽固醇，適閤于臨床常規及研究使用。
대경지당응효전영법동시측정혈청겁저밀도지단백(VLDL)、저밀도지단백(LDL)、고밀도지단백(HDL)화지단백(a)[LP(a)]적담고순[즉VLDL-C、LDL-C、HDL-C화LP(a)-C]함량적방법진행평개。방법채용Helena REP전자동쾌속전영계통분리혈청VLDL、LDL、HDL화LP(a)，연후결합담고순매염색법동시측정VLDL-C、LDL-C、HDL-C화LP(a)-C적함량。분석료해법적정밀도、준학도화간우인소，병장해법여전통방법여측정HDL-C적린오산-미침정법(PTA-Mg2＋법)、측정LDL-C적취을희류산침정법(PVS법)급측정LP(a)적면역투사비탁법(ITA법)진행비교。결과전영법측정VLDL-C、LDL-C화HDL-C적비내CV분별위5.16％～7.46％、1.26％～3.28％、3.78％～5.86％；비간CV분별위8.35％～11.25％、2.78％～4.08％、4.23％～6.36％。검측선성범위총담고순위1.04～10.35 mmol/L，기본불수용혈、황달화지혈간우。VLDL-C、LDL-C화HDL-C적평균회수솔분별위90.3％、94.3％화89.6％。전영법여전통법측정LDL-C、HDL-C적상관계수(r)분별위0.9609、0.9557。전영법측정LP(a)-C여ITA법측정LP(a)적r위0.9235。이해법검측북경지구98례건강인，HDL-C、VLDL-C、LDL-C、LP(a)-C삼고범위분별위0.95～2.10 mmol/L、0.07～0.46mmol/L、1.77～2.87 mmol/L、0～0.22 mmol/L。결론전영법측정치여전통법일치，전영법조작간편、쾌속경제，가동시측정4충지단백담고순，괄합우림상상규급연구사용。
Objective To evaluate a single step electrophoresis for quantitative determination of cholesterol of high-, low-, very-low-density lipoprotein(HDL, LDL, VLDL) and fast pre-beta lipoprotein [lipoprotein (a), Lp(a)]. Methods Quantification of lipoprotein cholesterol was performed by enzymatic staining of cholesterol in a new agarose gel electrophoresis method that allows the separation of LDL, VLDL, HDL, and Lp(a) by Helena REP system. The results of electrophoresis method were compared with those by traditional method like PTA-Mg2＋ precipitation method for HDL-C, PVS precipitation method for LDL-C, and Immunoturbidimetric assay(ITA) method for Lp(a).Results Within-runs CV were 5.16% ～ 7.46%,1.26% ～ 3.28% and 3.78% ～ 5.86% for VLDL-C, LDL-C and HDL-C, respectively. Between-runs CV were 8.35% ～ 11.25%, 2. 78% ～4. 08% and 4. 23% ～6. 36%, respectively. The linearity of this method was up to 10. 35 mmol/L total cholesterol. The recoveries were 90. 3%, 94. 3% and 89. 6%, respectively. No interference were observed when bilirubin( ＜ 342 μmol/L), hemoglobin( ＜ 20g/L) or triglyceride( ＜ 11.0 mmol/L) were added to pooled serum, respectively. There was good agreement between methods, with r = 0.9557 for HDL-C(electrophoresis method vs PTA-Mg2+ precipitation method),r = 0. 9609 for LDL-C (electrophoresis method vs PVS precipitation method) and r = 0. 9235 for Lp(a)-C (electrophoresis method) vs Lp(a) (ITA method). Conclusions The electrophoresis method offers a simple and inexpensive means of simultaneously measuring HDL-C, VLDL-C, Lp(a)-C and LDL-C.
