CAJ | 학술논문

本文通过透射电镜和扫描电镜观察，初步明确了果胶酶、纤维素酶及毒素对黄瓜叶组织超微结构的影响。在致病过程中，3种致病因子起着各自独立又相互联系的作用。3种致病因子对寄主超微结构的影响中，纤维素酶分解细胞壁能力最强，毒素对细胞质膜的作用最大，3种致病因子均可造成质壁分离，液泡、内质网受损。而叶绿体的被膜、片层结构主要被纤维素酶降解，线粒体的被膜主要被果胶酶降解，3种致病因子均能使叶绿体、线粒体内部空泡化。在分解叶表皮的过程中，所研究的3种致病因子首先是果胶酶降解果胶层，然后是纤维素酶、果胶酶、毒素对栅栏组织的分解，最后是纤维素酶、毒素作用于薄壁细胞壁，毒素、纤维素酶、果胶酶协同作用于细胞内部组织。
본문통과투사전경화소묘전경관찰，초보명학료과효매、섬유소매급독소대황과협조직초미결구적영향。재치병과정중，3충치병인자기착각자독립우상호련계적작용。3충치병인자대기주초미결구적영향중，섬유소매분해세포벽능력최강，독소대세포질막적작용최대，3충치병인자균가조성질벽분리，액포、내질망수손。이협록체적피막、편층결구주요피섬유소매강해，선립체적피막주요피과효매강해，3충치병인자균능사협록체、선립체내부공포화。재분해협표피적과정중，소연구적3충치병인자수선시과효매강해과효층，연후시섬유소매、과효매、독소대책란조직적분해，최후시섬유소매、독소작용우박벽세포벽，독소、섬유소매、과효매협동작용우세포내부조직。
The effects of the cellulase, pectinase and toxin produced by Cladosporium cucumerinum on the leaf cell of cucumber were studied by TEM and SEM. The results indicated that the three pathogenic factors played independent and symplastic roles. The cellulase and pectinase played leading roles respectively in decomposition of cell wall and plasma membrane. The three factors could all result in plasmolysis and damage vacuoles and endoplasmic reticulum. Chloroplast envelope and lamellae structure were mainly digested by cellulase, whiles, mitochondrion envelope was chiefly digested by the pectinase. Each of the three factors could cause vacuolation in chloroplast and mitochondria. During the process of breach up the structure of epidermis of leaf, the cellulase acted on reticulate region, then the pectinase dissolved pectic layer.Following this, palisade tissue was damaged by the cellulase, pectinase and toxin. Finally,parenchyma was destroyed by cellulase and toxin,successively.