CAJ | 학술논문

目的研究白细胞呼吸暴发化学发光活性与细胞形态行为变化特征及其相互关系，并探讨GbE对白细胞活化的干预作用。方法体外循环冠脉搭桥病人20例，采集转流前升主动脉血样，分离白细胞加入FMLP激活剂，孵化后置入超弱化学发光系统中，计算机描记白细胞呼吸暴发化学发光活性。根据发光曲线分为5个时段：0=未活化，1=活化初期，2=活化极期，3=活化后期，4=失活化。同步用布氏显微镜观察（放大1000倍），分析细胞形态行为变化特征,按这5个时段分为：A、B、C、D和E型5个细胞亚型，并作形态、功能相关分析。GbE（含黄酮苷0.545mg/ml)对白细胞呼吸暴发干预试验分为：对照组（白细胞＋FMLP）、GbE－1组（加入GbE5μl)、GbE－2组（加入GbE10μl)。结果1.FMLP激活白细胞呼吸暴发化学发光计数峰值明显升高，显示白细胞氧化暴发释放反应氧类活性的定量表达。2.白细胞活化的极期、后期发光活性与形态活化率呈正相关（r=0.91，0.75)，也与C型、D型细胞形态改变相一致。但活化初期B型细胞因缺乏脱颗粒释放，未能与发光计数平行上升。A型和E型细胞分别反映未活化和失活化状态均相符合。3.GbE对FMLP激活白细胞呼吸暴发化学发光活性有明显浓度依赖性抑制作用。活化极期GbE1组、GbE2组抑制百分率分别为：23.6％，53.35％(P<0.001)。结论FMLP激活白细胞呼吸暴发化学发光活性明显升高，表明白细胞释放自由基增加。布氏显微镜下细胞形态学可以区别白细胞未活化、活化和失活功能状态，为临床了解白细胞活性程度提供快速诊断方法。GbE对白细胞氧化暴发显示浓度依赖性抑制作用。
목적연구백세포호흡폭발화학발광활성여세포형태행위변화특정급기상호관계，병탐토GbE대백세포활화적간예작용。방법체외순배관맥탑교병인20례，채집전류전승주동맥혈양，분리백세포가입FMLP격활제，부화후치입초약화학발광계통중，계산궤묘기백세포호흡폭발화학발광활성。근거발광곡선분위5개시단：0=미활화，1=활화초기，2=활화겁기，3=활화후기，4=실활화。동보용포씨현미경관찰（방대1000배），분석세포형태행위변화특정,안저5개시단분위：A、B、C、D화E형5개세포아형，병작형태、공능상관분석。GbE（함황동감0.545mg/ml)대백세포호흡폭발간예시험분위：대조조（백세포＋FMLP）、GbE－1조（가입GbE5μl)、GbE－2조（가입GbE10μl)。결과1.FMLP격활백세포호흡폭발화학발광계수봉치명현승고，현시백세포양화폭발석방반응양류활성적정량표체。2.백세포활화적겁기、후기발광활성여형태활화솔정정상관（r=0.91，0.75)，야여C형、D형세포형태개변상일치。단활화초기B형세포인결핍탈과립석방，미능여발광계수평행상승。A형화E형세포분별반영미활화화실활화상태균상부합。3.GbE대FMLP격활백세포호흡폭발화학발광활성유명현농도의뢰성억제작용。활화겁기GbE1조、GbE2조억제백분솔분별위：23.6％，53.35％(P<0.001)。결론FMLP격활백세포호흡폭발화학발광활성명현승고，표명백세포석방자유기증가。포씨현미경하세포형태학가이구별백세포미활화、활화화실활공능상태，위림상료해백세포활성정도제공쾌속진단방법。GbE대백세포양화폭발현시농도의뢰성억제작용。
Objective. To study the chemiluminescence activity and morphology of leukocytes during respiratory burst and intervention by Ginko biloba extract (GbE).Methods 20 patients undergoing extracorporal circulation (ECC) were observed. Purified leukocyte, blood sample collected from ascending aorta before ECC turning flow. It was stimulated with FMLP and was incubated. Then, described the chemiluminescence activity curve by computer system. The curve can be divided into 0,1,2,3,4 five periods . Meanwhile, at each period, observed the morphology of the leukocytes which can be defined as A,B,C,D and E type. In the GbE groups, additional GbE (GbE－ 1 group 5ul. GbE－ 2 group 10ul) was added into the leukocyte samples than the above_mentioned control group.Results The leukocytes released a vast amount of reactive oxygen species (ROS) during respiratory burst; the C type leukocytes' activated rate and the chemiluminescence activity at the curve peak was markedly positive related (r=0.91, P<0.01).Conclusions Bradford variable projective microscopy (BVPM) provides a new rapid diagnostic method for leukocyte activation.There are concentration－ dependant inhibition, in chemiluminescence produced by activated leukocyte with FMLP. Additional GBE inhibited percentage showed 23.6％ (GBE－ 1) and 53.35％ (GBE－ 2) compared with control group(P<0.01).