CAJ | 학술논문

继代培养期间的烟草愈伤组织总呼吸速率分别在第11天和第19天出现两次跃升，同时抗氰呼吸的发生与运行逐渐加强，在两呼吸高峰之间达到极大值，随后则逐渐下降，但愈伤组织的电子传递仍以细胞色素途径为主．通过对愈伤组织衰老过程中活性氧代谢变化分析，发现抗氰呼吸发生与H2O2含量，其实际运行则与O-2含量呈平行关系，但交替途径容量的降低则与·OH含量显著上升紧密相关，用活性氧清除剂处理愈伤组织也得到上述相似的结果．由此推测愈伤组织在衰老过程中产生的活性氧诱导抗氰呼吸参与烟草愈伤组织起始衰老．
계대배양기간적연초유상조직총호흡속솔분별재제11천화제19천출현량차약승，동시항청호흡적발생여운행축점가강，재량호흡고봉지간체도겁대치，수후칙축점하강，단유상조직적전자전체잉이세포색소도경위주．통과대유상조직쇠로과정중활성양대사변화분석，발현항청호흡발생여H2O2함량，기실제운행칙여O-2함량정평행관계，단교체도경용량적강저칙여·OH함량현저상승긴밀상관，용활성양청제제처리유상조직야득도상술상사적결과．유차추측유상조직재쇠로과정중산생적활성양유도항청호흡삼여연초유상조직기시쇠로．
During the growth and senescence of tobacco callus, the total respiration rate showed two peaks on the 11th and 19th day, respectively. The development and operation of alternative pathway increased gradually, reached the maximum between two respiration peaks, and then declined in the following days, but the cytochrome pathway was still as a main electron transport pathway consistently. Analysis on the levels of active oxygen species during the growth and senescence process in tobacco callus suggested that H2O2 and O-2 were involved in some way in the induction of the development and operation of the alternative pathway, but the increase of production rate of OH might suppress both the development and operation of the alternative pathway, which were enhanced by H2O2 and O-2 respectively. These results were further proved by the use of active oxygen scavengers. The possible participation of the alternative pathway enhanced by active oxygen in the initial senescence process was discussed.