云南植物研究
雲南植物研究
운남식물연구
ACTA BOTANICA YUNNANICA
2001年
1期
91-96
,共6页
离体茎段嫁接%植物激素%维管组织
離體莖段嫁接%植物激素%維管組織
리체경단가접%식물격소%유관조직
植物离体茎段嫁接系统是在无菌条件下将茎切段嫁接后放入培养基中，使接穗和砧木分别与含不同成分的培养基接触，再置光下培养的一个模拟植物正常生理过程、环境条件可控的实验系统。离体茎段嫁接体的发育与整体类似，包括接穗与砧木粘连、愈伤组织产生、次生胞间连丝形成和维管束分化等几个步骤，发育进程受植物激素如生长素和细胞分裂素调节。该系统的建立为阐明嫁接体发育机理及嫁接亲和性机制提供了重要的依据。
植物離體莖段嫁接繫統是在無菌條件下將莖切段嫁接後放入培養基中，使接穗和砧木分彆與含不同成分的培養基接觸，再置光下培養的一箇模擬植物正常生理過程、環境條件可控的實驗繫統。離體莖段嫁接體的髮育與整體類似，包括接穗與砧木粘連、愈傷組織產生、次生胞間連絲形成和維管束分化等幾箇步驟，髮育進程受植物激素如生長素和細胞分裂素調節。該繫統的建立為闡明嫁接體髮育機理及嫁接親和性機製提供瞭重要的依據。
식물리체경단가접계통시재무균조건하장경절단가접후방입배양기중，사접수화침목분별여함불동성분적배양기접촉，재치광하배양적일개모의식물정상생리과정、배경조건가공적실험계통。리체경단가접체적발육여정체유사，포괄접수여침목점련、유상조직산생、차생포간련사형성화유관속분화등궤개보취，발육진정수식물격소여생장소화세포분렬소조절。해계통적건립위천명가접체발육궤리급가접친화성궤제제공료중요적의거。
The grafting of excised hypocotyl segment of plant in sterileculture is described in which two explanted internodes are grafted together in sterile culture and then inserted between two agar surfaces and incubated under light. It imitates the normal physiological process, and is a controllable experimental system. Development of the graft union is similar to that observed in whole-plant graft unions, including a sequence of several distinct steps: initial cohesion of stock and scion; proliferation of callus at graft interface; symplastic interconnection of individual cells of graft partners by secondarily formed plasmodesmata; and vascular bridge formation. The process of development is regulated by plant hormones such as auxin and cytokinin. Clearly this system can be used as a powerful tool in demonstrating the mechanisms of graft formation and graft compatibility.
