临床耳鼻咽喉科杂志
臨床耳鼻嚥喉科雜誌
림상이비인후과잡지
LINCHUANG ER-BL-YANHOUKE ZAZHL
2001年
1期
26-27
,共2页
郭英%苏振伦%杨伟炎%姜泗长
郭英%囌振倫%楊偉炎%薑泗長
곽영%소진륜%양위염%강사장
耳蜗%外毛细胞%细胞分离
耳蝸%外毛細胞%細胞分離
이와%외모세포%세포분리
目的：探讨豚鼠耳蜗各回外毛细胞（OHC）的分离方法。方法：选豚鼠8只，解剖耳蜗各回组织，获得Corti器并采用酶消化后机械分离。结果：各回均可获得一定数量、活性良好的OHC，第1、2、3、4回单离OHC的长度依次为23.81、34.50、60.48和71.37 μm。结论：熟悉耳蜗各回解剖、组织特性并按操作要点进行是成功分离出各回OHC的关键。
目的：探討豚鼠耳蝸各迴外毛細胞（OHC）的分離方法。方法：選豚鼠8隻，解剖耳蝸各迴組織，穫得Corti器併採用酶消化後機械分離。結果：各迴均可穫得一定數量、活性良好的OHC，第1、2、3、4迴單離OHC的長度依次為23.81、34.50、60.48和71.37 μm。結論：熟悉耳蝸各迴解剖、組織特性併按操作要點進行是成功分離齣各迴OHC的關鍵。
목적：탐토돈서이와각회외모세포（OHC）적분리방법。방법：선돈서8지，해부이와각회조직，획득Corti기병채용매소화후궤계분리。결과：각회균가획득일정수량、활성량호적OHC，제1、2、3、4회단리OHC적장도의차위23.81、34.50、60.48화71.37 μm。결론：숙실이와각회해부、조직특성병안조작요점진행시성공분리출각회OHC적관건。
Objective:To probe the method of isolating outer hair cells (OHC) from each of four turns of the guinea-pig cochlea. Method:From eight guinea pigs the organ of Corti from each of four turns of the cochlea were dissected, and then treated using enzyme. Result:A fair amount of living OHCs from each of four turns were obtained. The length of OHCs from each of four turns were 23.81,34.50,60.48 and 71.37 μm. Conclusion:The key to success in isolating OHCs from each of four turns of the cochlea is to know very well the anatomical characteristics of each of four turns of the cochlea and be operated in accordance with normal rules.
