CAJ | 학술논문

目的：探讨内皮依赖性舒张因子(EDRF／NO)对豚鼠耳蜗微循环的调节作用。方法：28只豚鼠，随即分为4组，每组7只。生理盐水组静脉注射生理盐水，Ｌ-Arg／S组和L-Arg／L组静脉注射不同剂量L-精氨酸，L-NNA组静脉注射L-硝基-精氨酸。行耳蜗开窗后，利用计算机图像分析系统，对豚鼠耳蜗的血流、血管管径、血压、心率、血气进行分析。观察了不同剂量L-精氨酸(L-Arg)及L-硝基-精氨酸(L-NNA)对豚鼠耳蜗血流(CoBF)的作用。结果：L-Arg／S组，用药后耳蜗血液流速加快，管径扩张，平均动脉压均下降；L-Arg／L组，用药后耳蜗血流速度增加后流速减慢，管径扩张，血压升高；L-NNA组，用药后流速减慢，血管管径收缩，但持续时间很短。与用药前相比差异均有显著性(P＜0.05)。结论：EDRF/NO对CoBF有调节作用，适当剂量的EDRF/NO可扩张血管，加快血流，增加耳蜗微循环的灌注量。
목적：탐토내피의뢰성서장인자(EDRF／NO)대돈서이와미순배적조절작용。방법：28지돈서，수즉분위4조，매조7지。생리염수조정맥주사생리염수，Ｌ-Arg／S조화L-Arg／L조정맥주사불동제량L-정안산，L-NNA조정맥주사L-초기-정안산。행이와개창후，이용계산궤도상분석계통，대돈서이와적혈류、혈관관경、혈압、심솔、혈기진행분석。관찰료불동제량L-정안산(L-Arg)급L-초기-정안산(L-NNA)대돈서이와혈류(CoBF)적작용。결과：L-Arg／S조，용약후이와혈액류속가쾌，관경확장，평균동맥압균하강；L-Arg／L조，용약후이와혈류속도증가후류속감만，관경확장，혈압승고；L-NNA조，용약후류속감만，혈관관경수축，단지속시간흔단。여용약전상비차이균유현저성(P＜0.05)。결론：EDRF/NO대CoBF유조절작용，괄당제량적EDRF/NO가확장혈관，가쾌혈류，증가이와미순배적관주량。
Aim: To investigate the role of endothelium-derived relaxing factor(EDRF/NO)in regulating cochlear microcircu-latian. Methods: The total of 28 guinea pigs were randomly divided into 4 groups (7 guinea pigs each group). Normal saline groupwas injected with normal saline. L-Arg/S group and L-Arg/L group were injected with L-Arg. L-NNA group was injected with L-NNA. After cochlear fenestration was done with intravital micreseopic examination, cochlear blood flow(CoBF), vessel diameter,blood pressure of guinea pigs were analysed using image processing technique. Results: In L-Arg/S group CoBF increased, micro-vessel was dilated, and mean arterial pressure declined; In L-Arg/L group CoBF increased, and slackened later, micro-vessel wasdilated, mean arterial pressure was elevated; In L-NNA group CoBF slackened , micro-vessel contracted. In all 3 groups therewere significant difference compared with those before administration( P < 0.05). Conclusion: CoBF can be regulated by EDRF/NO in guinea pigs. EDRF/NO can be used to treat inner ear disorder by dialating vessel and increasing blood velocity.