CAJ | 학술논문

目的　研究大鼠胰腺促性腺激素释放激素(GnRH)受体mRNA的存在及细胞定位. 方法　采用原位杂交组织化学法. 结果　胰脏的外分泌部腺上皮细胞、胰导管上皮细胞及内分泌部胰岛细胞内均可检测到较强的GnRH受体mRNA杂交信号. 杂交阳性信号物质均分布在胞质内，胞核呈阴性. 结论　大鼠胰脏内、外分泌部均能合成GnRH受体. GnRH也是一种消化激素，它由胰脏自身合成，又作用于胰脏,可能参与胰脏内、外分泌功能的调节.
목적　연구대서이선촉성선격소석방격소(GnRH)수체mRNA적존재급세포정위. 방법　채용원위잡교조직화학법. 결과　이장적외분비부선상피세포、이도관상피세포급내분비부이도세포내균가검측도교강적GnRH수체mRNA잡교신호. 잡교양성신호물질균분포재포질내，포핵정음성. 결론　대서이장내、외분비부균능합성GnRH수체. GnRH야시일충소화격소，타유이장자신합성，우작용우이장,가능삼여이장내、외분비공능적조절.
AIM　To study whether gonadotropin releasing hormone receptor (GnRHR) mRNA exists in rat pancreas and its cellular localization. METHODS　In situ hybridization with digoxigenin-labeled oligonucleotide probes was adopted. RESULTS　Pancreatic endocrine islet cells, exocrine glandular cells and exocrine duct epithelial cells were all found to have GnRHR mRNA hybridization signals. The signals distributed in cytoplasm of all positive cells with negative nucli. CONCLUSION　Both of the exocrine and endocrine cells of the pancrea can produce GnRH recepor. GnRH is also a kind of digestive hormone produced by pancrea and is responsible for modulating the exocrine and endocrine function.