CAJ | 학술논문

辛纳毒蛋白是从香樟种子中分离的一种Ⅱ核糖体失活蛋白.最近,从香樟种子中还分离到另一种微型双链核糖体失活蛋白,命名为新丰毒蛋白.还原的新丰毒蛋白表现出与还原的辛纳毒蛋白同样的RNA N-糖苷酶和体外对抑制蛋白质翻译的活力.新丰毒蛋白的B链与辛纳毒蛋白的B链具有同样的分子质量和相同的N端10个氨基酸序列.它的A链N端10个氨基酸序列也与辛纳毒蛋白的A链完全一致,并且C端与辛纳毒蛋白的A链一样具有半胱氨酸,但是它的分子质量却只有辛纳毒蛋白A链的一半.RT-PCR和RNA印迹结果表明体内不存在新丰毒蛋白的mRNA.推测新丰毒蛋白是从辛纳毒蛋白通过蛋白质剪接而产生的,是一种研究蛋白质剪接的好材料.
신납독단백시종향장충자중분리적일충Ⅱ핵당체실활단백.최근,종향장충자중환분리도령일충미형쌍련핵당체실활단백,명명위신봉독단백.환원적신봉독단백표현출여환원적신납독단백동양적RNA N-당감매화체외대억제단백질번역적활력.신봉독단백적B련여신납독단백적B련구유동양적분자질량화상동적N단10개안기산서렬.타적A련N단10개안기산서렬야여신납독단백적A련완전일치,병차C단여신납독단백적A련일양구유반광안산,단시타적분자질량각지유신납독단백A련적일반.RT-PCR화RNA인적결과표명체내불존재신봉독단백적mRNA.추측신봉독단백시종신납독단백통과단백질전접이산생적,시일충연구단백질전접적호재료.
Cinnamomin is a type Ⅱ ribosome-inactivating protein (RIP) isolated from the seed of camphor tree (Cinnamomum camphora). Recently, a small RIP named as cinphorin was isolated from the seed of camphor tree. The reduced cinphorin exhibited the RNA N-glycosidase activity and inhibitory activity to protein synthesis in vitro like the reduced cinnamomin did. Cinphorin B-chain exhibited the same N-terminal 10 amino acid sequence and molecular mass as the B-chain of cinnamomin. Its A-chain exhibited the same 10 N-terminal amino acid sequence and owned a C-terminal cysteine residue linked to the B-chain, but the molecular mass of cinphorin A-chain was only half of cinnamomin A-chain. RT-PCR and Northern blotting revealed that there was no corresponding mRNA of cinphorin. Cinphorin is probably produced from cinnamomin by protein splicing.