北京大学学报(医学版)
北京大學學報(醫學版)
북경대학학보(의학판)
JOURNAL OF PEAKING UNIVERSITY(HEALTH SCIENCES)
2002年
2期
122-132
,共11页
金力坚%梁惠强%E.F.Corbet%L.P.Samaranayake%W.I.R.Davies%P.O.Soder
金力堅%樑惠彊%E.F.Corbet%L.P.Samaranayake%W.I.R.Davies%P.O.Soder
금력견%량혜강%E.F.Corbet%L.P.Samaranayake%W.I.R.Davies%P.O.Soder
中性粒细胞弹力酶%龈沟液/细胞学%牙周炎/病理学
中性粒細胞彈力酶%齦溝液/細胞學%牙週炎/病理學
중성립세포탄력매%간구액/세포학%아주염/병이학
Granulocyte elastase%Gingival crevicular fluid/cytol%Chronic periodontitis/pathol
目的:研究人类龈沟液中性粒细胞弹力酶的动态活动方式和最高活性率,从而探讨其作为一项定量检测牙周状况指标的可能性.方法:利用滤纸条法,从33例慢性牙周炎患者提取217份龈沟液样本,用中性粒细胞弹力酶的特异性底物对该酶在龈沟液中的活性进行动态定量测量,从而计算该酶最高活性率(MR-EA,单位为每个牙部位mAbs*min-1),并将结果与临床牙周状况进行综合比较分析.结果:龈沟液中性粒细胞弹力酶可分为5种不同的酶活动方式(A,B,C,D,E),其相应的MR-EA存在显著差异(P＜0.01).在临床牙周状况相同的牙部位,弹力酶活动差异有显著性,各种临床部位的弹力酶活动方式高(A%+B%)/低(D%+E%)分布情况如下:健康部位0%/92%,牙龈炎部位6%/72%和牙周炎部位21%/54%.健康部位的MR-EA值≤3.0,而病变部位的MR-EA值存在明显差异,具体分布如下:75%牙龈炎部位的MR-EA≤3.0,13%＞6.0,4%＞10.0;而60%牙周炎部位的MR-EA值≤3.0,30%＞6.0,21%＞10.0.结论:MR-EA能作为定量测定龈沟液中性粒细胞弹力酶活动的适当指标,可以用来定量检测牙周状况.
目的:研究人類齦溝液中性粒細胞彈力酶的動態活動方式和最高活性率,從而探討其作為一項定量檢測牙週狀況指標的可能性.方法:利用濾紙條法,從33例慢性牙週炎患者提取217份齦溝液樣本,用中性粒細胞彈力酶的特異性底物對該酶在齦溝液中的活性進行動態定量測量,從而計算該酶最高活性率(MR-EA,單位為每箇牙部位mAbs*min-1),併將結果與臨床牙週狀況進行綜閤比較分析.結果:齦溝液中性粒細胞彈力酶可分為5種不同的酶活動方式(A,B,C,D,E),其相應的MR-EA存在顯著差異(P＜0.01).在臨床牙週狀況相同的牙部位,彈力酶活動差異有顯著性,各種臨床部位的彈力酶活動方式高(A%+B%)/低(D%+E%)分佈情況如下:健康部位0%/92%,牙齦炎部位6%/72%和牙週炎部位21%/54%.健康部位的MR-EA值≤3.0,而病變部位的MR-EA值存在明顯差異,具體分佈如下:75%牙齦炎部位的MR-EA≤3.0,13%＞6.0,4%＞10.0;而60%牙週炎部位的MR-EA值≤3.0,30%＞6.0,21%＞10.0.結論:MR-EA能作為定量測定齦溝液中性粒細胞彈力酶活動的適噹指標,可以用來定量檢測牙週狀況.
목적:연구인류간구액중성립세포탄력매적동태활동방식화최고활성솔,종이탐토기작위일항정량검측아주상황지표적가능성.방법:이용려지조법,종33례만성아주염환자제취217빈간구액양본,용중성립세포탄력매적특이성저물대해매재간구액중적활성진행동태정량측량,종이계산해매최고활성솔(MR-EA,단위위매개아부위mAbs*min-1),병장결과여림상아주상황진행종합비교분석.결과:간구액중성립세포탄력매가분위5충불동적매활동방식(A,B,C,D,E),기상응적MR-EA존재현저차이(P＜0.01).재림상아주상황상동적아부위,탄력매활동차이유현저성,각충림상부위적탄력매활동방식고(A%+B%)/저(D%+E%)분포정황여하:건강부위0%/92%,아간염부위6%/72%화아주염부위21%/54%.건강부위적MR-EA치≤3.0,이병변부위적MR-EA치존재명현차이,구체분포여하:75%아간염부위적MR-EA≤3.0,13%＞6.0,4%＞10.0;이60%아주염부위적MR-EA치≤3.0,30%＞6.0,21%＞10.0.결론:MR-EA능작위정량측정간구액중성립세포탄력매활동적괄당지표,가이용래정량검측아주상황.
Objective: To investigate the dynamic pattern of granulocyte-specific elastase activity in gingival crevicular fluid (GCF) from subjects with chronic periodontitis, and to evaluate whether this assay could serve for assessment of periodontal conditions. Methods: GCF was collected by paper strips at 217 sites from 33 subjects with untreated chronic periodontitis. Dynamics of the elastase activities in GCF were analyzed with a substrate specific for granulocyte elastase, L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide. Results: Five time-dependent, dynamic patterns of elastase activity were defined and named Patterns A to E. The highest slope portion of a plotted substrate hydrolysis time course within a 5-h period was used to calculate the maximal rate of elastase activity (MR-EA, mAbs/min/site), while the maximal level of elastase activity (M-EA, Abs/site) within that period was also calculated for comparison. Marked differences were found in MR-EA among the five patterns (P＜0.01), while measuring M-EA alone failed to distinguish between Patterns A and B. Among these sites with high GCF volume and those within varying categories of probing depths with or without bleeding on probing, a large variation in elastase activity was observed. High/low activity patterns, i.e., A & B/D & E, were found respectively in 0%/92% of the healthy sites, 6%/72% of the gingivitis sites, and 21%/54% of the periodontitis sites. A wide range of MR-EA values was found among the diseased sites when a maximal MR-EA value for healthy sites was delineated at 3.0. Of the gingivitis sites, 75% had values ≤3.0,13%＞6.0,and 4%＞10.0. The corresponding values for periodontitis sites were 60%, 30% and 21%, respectively. Conclusion: MR-EA seems to be an appropriate measurement parameter for assay of GCF granulocyte elastase activity. MR-EA may thus serve as a useful host marker for quantitative assessment of periodontal conditions.
